System Modularity Chip for Analysis of Rare Targets (SMART-Chip): Liquid Biopsy Samples.

Autor:	Pahattuge TN; Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, Kansas 66045, United States.; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States., Freed IM; Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, Kansas 66045, United States.; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States., Hupert ML; BioFluidica, Inc., 1567 Irving Hill Road, Lawrence, Kansas 66045, United States., Vaidyanathan S; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States.; Department of BioEngineering, University of Kansas, 1530 West 15th Street, Lawrence, Kansas 66045, United States., Childers K; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States.; Department of BioEngineering, University of Kansas, 1530 West 15th Street, Lawrence, Kansas 66045, United States., Witek MA; Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, Kansas 66045, United States.; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States., Weerakoon-Ratnayake K; Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, Kansas 66045, United States.; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States., Park D; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States.; Department of Mechanical & Industrial Engineering, Louisiana State University, 3261 Patrick F. Taylor Hall, Baton Rouge, Louisiana 70803, United States., Kasi A; Department of Medical Oncology, University of Kansas Medical Center, Kansas City, Kansas 66160, United States., Al-Kasspooles MF; Department of Medical Oncology, University of Kansas Medical Center, Kansas City, Kansas 66160, United States., Murphy MC; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States.; Department of Mechanical & Industrial Engineering, Louisiana State University, 3261 Patrick F. Taylor Hall, Baton Rouge, Louisiana 70803, United States., Soper SA; Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, Kansas 66045, United States.; Center of BioModular Multi-scale Systems for Precision Medicine, University of Kansas, Lawrence, Kansas 66045, United States.; BioFluidica, Inc., 1567 Irving Hill Road, Lawrence, Kansas 66045, United States.; Department of BioEngineering, University of Kansas, 1530 West 15th Street, Lawrence, Kansas 66045, United States.; Department of Mechanical Engineering, University of Kansas, 3138 Learned Hall, 1530 West 15th Street, Lawrence, Kansas 66045, United States.; Department of Cancer Biology and KU Cancer Center, University of Kansas Medical Center, Kansas City, Kansas 66160, United States.
Jazyk:	angličtina
Zdroj:	ACS sensors [ACS Sens] 2021 May 28; Vol. 6 (5), pp. 1831-1839. Date of Electronic Publication: 2021 May 03.
DOI:	10.1021/acssensors.0c02728
Abstrakt:	Liquid biopsies are becoming popular for managing a variety of diseases due to the minimally invasive nature of their acquisition, thus potentially providing better outcomes for patients. Circulating tumor cells (CTCs) are among the many different biomarkers secured from a liquid biopsy, and a number of efficient platforms for their isolation and enrichment from blood have been reported. However, many of these platforms require manual sample handling, which can generate difficulties when translating CTC assays into the clinic due to potential sample loss, contamination, and the need for highly specialized operators. We report a system modularity chip for the analysis of rare targets (SMART-Chip) composed of three task-specific modules that can fully automate processing of CTCs. The modules were used for affinity selection of the CTCs from peripheral blood with subsequent photorelease, simultaneous counting, and viability determinations of the CTCs and staining/imaging of the CTCs for immunophenotyping. The modules were interconnected to a fluidic motherboard populated with valves, interconnects, pneumatic control channels, and a fluidic network. The SMART-Chip components were made from thermoplastics via microreplication, which lowers the cost of production making it amenable to clinical implementation. The utility of the SMART-Chip was demonstrated by processing blood samples secured from colorectal cancer (CRC) and pancreatic ductal adenocarcinoma (PDAC) patients. We were able to affinity-select EpCAM expressing CTCs with high purity (0-3 white blood cells/mL of blood), enumerate the selected cells, determine their viability, and immunophenotype the cells. The assay could be completed in <4 h, while manual processing required >8 h.
Databáze:	MEDLINE
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