Phage-display reveals interaction of lipocalin allergen Can f 1 with a peptide resembling the antigen binding region of a human γδT-cell receptor.
| Autor: | Habeler M; Institute of Molecular Biology, Medical University Innsbruck, Innrain 80, A-6020 Innsbruck, Austria., Redl B; Institute of Molecular Biology, Medical University Innsbruck, Innrain 80, A-6020 Innsbruck, Austria. |
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| Jazyk: | angličtina |
| Zdroj: | Biological chemistry [Biol Chem] 2020 Sep 16; Vol. 402 (4), pp. 433-437. Date of Electronic Publication: 2020 Sep 16 (Print Publication: 2021). |
| DOI: | 10.1515/hsz-2020-0185 |
| Abstrakt: | Although some progress has been achieved in understanding certain aspects of the allergenic mechanism of animal lipocalins, they still remain largely enigmatic. One possibility to unravel this property is to investigate their interaction with components of the immune system. Since these components are highly complex we intended to use a high-throughput technology for this purpose. Therefore, we used phage-display of a random peptide library for panning against the dog allergen Can f 1. By this method we identified a Can f 1 binding peptide corresponding to the antigen-binding site of a putative γδT-cell receptor. Additional biochemical investigations confirmed this interaction. (© 2020 Matthias Habeler and Bernhard Redl, published by De Gruyter, Berlin/Boston.) |
| Databáze: | MEDLINE |
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