Experimental and natural evidence of SARS-CoV-2-infection-induced activation of type I interferon responses.

Autor:	Banerjee A; Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada., El-Sayes N; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., Budylowski P; Institute of Medical Science, University of Toronto, Toronto, ON M5S 1A8, Canada., Jacob RA; Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada., Richard D; Department of Human Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Maan H; Vector Institute for Artificial Intelligence, Toronto, ON M5G 1M1, Canada.; Peter Munk Cardiac Centre, University Health Network, Toronto, ON M5G 2C4, Canada., Aguiar JA; Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada., Demian WL; Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada., Baid K; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., D'Agostino MR; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., Ang JC; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., Murdza T; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., Tremblay BJ; Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada., Afkhami S; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., Karimzadeh M; Vector Institute for Artificial Intelligence, Toronto, ON M5G 1M1, Canada., Irving AT; Zhejiang University - University of Edinburgh Institute, Haining, Zhejiang 314400, China.; Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310027, China., Yip L; Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada., Ostrowski M; Department of Medicine, University of Toronto, Toronto, ON M5S 3H2, Canada.; Keenan Research Centre for Biomedical Science of St. Michael's Hospital, UnityHealth, Toronto, ON M5B 1W8, Canada., Hirota JA; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; Division of Respirology, Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.; Division of Respiratory Medicine, The University of British Columbia, Vancouver, BC V5Z 1M9, Canada., Kozak R; Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada., Capellini TD; Department of Human Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA., Miller MS; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., Wang B; Vector Institute for Artificial Intelligence, Toronto, ON M5G 1M1, Canada.; Peter Munk Cardiac Centre, University Health Network, Toronto, ON M5G 2C4, Canada.; Department of Computer Science, University of Toronto, Toronto, ON M5S 2E4, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada., Mubareka S; Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada., McGeer AJ; Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada.; Dalla Lana School of Public Health, University of Toronto, Toronto, ON M5S 1A1, Canada., McArthur AG; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada., Doxey AC; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; Department of Biology, University of Waterloo, Waterloo, ON N2L 3G1, Canada., Mossman K; Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.; Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, ON L8S 4K1, Canada.; McMaster Immunology Research Centre, McMaster University, Hamilton, ON L8S 4K1, Canada.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2021 May 21; Vol. 24 (5), pp. 102477. Date of Electronic Publication: 2021 Apr 26.
DOI:	10.1016/j.isci.2021.102477
Abstrakt:	Type I interferons (IFNs) are our first line of defense against virus infection. Recent studies have suggested the ability of SARS-CoV-2 proteins to inhibit IFN responses. Emerging data also suggest that timing and extent of IFN production is associated with manifestation of COVID-19 severity. In spite of progress in understanding how SARS-CoV-2 activates antiviral responses, mechanistic studies into wild-type SARS-CoV-2-mediated induction and inhibition of human type I IFN responses are scarce. Here we demonstrate that SARS-CoV-2 infection induces a type I IFN response in vitro and in moderate cases of COVID-19. In vitro stimulation of type I IFN expression and signaling in human airway epithelial cells is associated with activation of canonical transcriptions factors, and SARS-CoV-2 is unable to inhibit exogenous induction of these responses. Furthermore, we show that physiological levels of IFNα detected in patients with moderate COVID-19 is sufficient to suppress SARS-CoV-2 replication in human airway cells. Competing Interests: The authors declare no competing interests. (© 2021 The Author(s).)
Databáze:	MEDLINE
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