Comparative evaluation of the immunodominant proteins of Brucella abortus for the diagnosis of cattle brucellosis.

Autor: Nagalingam M; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Basheer TJ; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Balamurugan V; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Shome R; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Kumari SS; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Reddy GBM; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Shome BR; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Rahman H; International Livestock Research Institute, New Delhi, India., Roy P; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India., Kingston JJ; Defense Food Research Laboratory, Mysore, Karnataka, India., Gandham RK; National Institute of Animal Biotechnology, Hyderabad, Telangana, India.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2021 Mar; Vol. 14 (3), pp. 803-812. Date of Electronic Publication: 2021 Mar 30.
DOI: 10.14202/vetworld.2021.803-812
Abstrakt: Background and Aim: The present serodiagnosis of brucellosis in livestock is based on the whole cell or smooth lipopolysaccharide of the Brucella organism in which specificity is hampered by the cross-reactivity, especially with the antibodies against Yersinia enterocolitica O:9 organism. The problem can be addressed by screening for better immunodominant antigens. Hence, the present study was undertaken to screen protein antigens of Brucella abortus for their diagnostic potential in cattle brucellosis.
Materials and Methods: Protein antigens of B. abortus (n=10) non-reactive to antibodies against Y. enterocolitica O:9 were selected, expressed in Escherichia coli , assessed the reactivity of expressed recombinant proteins by Western blot, standardized indirect-enzyme-linked immunosorbent assay (ELISA) for detecting Brucella antibodies in cattle serum, and comparative evaluation was done.
Results: All the selected protein antigens were expressed and in the Western blot with Brucella antibodies positive cattle serum, six recombinant ( Brucella protein 26 [BP26], Cu-Zn Superoxide dismutase [SodC], B. abortus I-1885, Serine protease, Bacterioferritin, and Brucella Lumazine Synthase [BLS]) proteins showed reaction whereas none of the proteins showed reactivity with Brucella negative cattle serum. ELISA has been done using known Brucella positive and negative cattle sera samples (n=113 each) in which the performance of recombinant proteins in diagnosing brucellosis was in the order of BP26 > BLS > SodC followed by rest of the proteins. BP26 based ELISA was found to be better with area under the curve as 0.953, and diagnostic sensitivity, diagnostic specificity, and Youden's index of 90.27%, 95.58%, and 0.8584, respectively, with the excellent agreement (k=0.85).
Conclusion: BP26 could be a potential diagnostic antigen among the immunodominant proteins of B. abortus in ruling out Y. enterocolitica O:9 infection while diagnosing brucellosis in cattle herds.
(Copyright: © Nagalingam, et al.)
Databáze: MEDLINE
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