Metabolic changes in the brain and blood of rats following acoustic trauma, tinnitus and hyperacusis.

Autor:	He J; Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu, China; Department of Pharmacology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China., Aa JY; Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu, China., Sun JG; Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu, China., Smith PF; Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand; Brain Health Research Centre, University of Otago, Dunedin, New Zealand; Brain Research New Zealand, Dunedin, New Zealand; Eisdell Moore Centre for Hearing and Balance Research, University of Auckland, Auckland, New Zealand., De Ridder D; Brain Health Research Centre, University of Otago, Dunedin, New Zealand; Brain Research New Zealand, Dunedin, New Zealand; Eisdell Moore Centre for Hearing and Balance Research, University of Auckland, Auckland, New Zealand; Department of Surgical Sciences, Section of Neurosurgery, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand., Wang GJ; Jiangsu Province Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu, China. Electronic address: guangjiwang@hotmail.com., Zheng Y; Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand; Brain Health Research Centre, University of Otago, Dunedin, New Zealand; Brain Research New Zealand, Dunedin, New Zealand; Eisdell Moore Centre for Hearing and Balance Research, University of Auckland, Auckland, New Zealand. Electronic address: yiwen.zheng@otago.ac.nz.
Jazyk:	angličtina
Zdroj:	Progress in brain research [Prog Brain Res] 2021; Vol. 262, pp. 399-430. Date of Electronic Publication: 2021 Jan 19.
DOI:	10.1016/bs.pbr.2020.09.002
Abstrakt:	It has been increasingly recognized that tinnitus is likely to be generated by complex network changes. Acoustic trauma that causes tinnitus induces significant changes in multiple metabolic pathways in the brain. However, it is not clear whether those metabolic changes in the brain could also be reflected in blood samples and whether metabolic changes could discriminate acoustic trauma, hyperacusis and tinnitus. We analyzed brain and serum metabolic changes in rats following acoustic trauma or a sham procedure using metabolomics. Hearing levels were recorded before and after acoustic trauma and behavioral measures to quantify tinnitus and hyperacusis were conducted at 4 weeks following acoustic trauma. Tissues from 11 different brain regions and serum samples were collected at about 3 months following acoustic trauma. Among the acoustic trauma animals, eight exhibited hyperacusis-like behavior and three exhibited tinnitus-like behavior. Using Gas chromatography-mass spectrometry and multivariate statistical analysis, significant metabolic changes were found in acoustic trauma animals in both the brain and serum samples with a number of metabolic pathways significantly perturbated. Furthermore, metabolic changes in the serum were able to differentiate sham from acoustic trauma animals, as well as sham from hyperacusis animals, with high accuracy. Our results suggest that serum metabolic profiling in combination with machine learning analysis may be a promising approach for identifying biomarkers for acoustic trauma, hyperacusis and potentially, tinnitus. (Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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