UPLC-PDA-ESI-QTOF-MS/MS fingerprint of purified flavonoid enriched fraction of Bryophyllum pinnatum ; antioxidant properties, anticholinesterase activity and in silico studies.

Autor: Ogidigo JO; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria.; Bioresources Development Centre, National Biotechnology Development Agency, Abuja, Nigeria., Anosike CA; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Joshua PE; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Ibeji CU; Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria., Ekpo DE; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Nwanguma BC; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Nwodo OFC; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria.; Department of Biochemistry, Mkar University, Benue State, Nigeria.
Jazyk: angličtina
Zdroj: Pharmaceutical biology [Pharm Biol] 2021 Dec; Vol. 59 (1), pp. 444-456.
DOI: 10.1080/13880209.2021.1913189
Abstrakt: Context: Bryophyllum pinnatum (Lam.) Oken (Crassulaceae) is used traditionally to treat many ailments.
Objectives: This study characterizes the constituents of B. pinnatum flavonoid-rich fraction (BPFRF) and investigates their antioxidant and anticholinesterase activity using in vitro and in silico approaches.
Materials and Methods: Methanol extract of B. pinnatum leaves was partitioned to yield the ethyl acetate fraction. BPFRF was isolated from the ethyl acetate fraction and purified. The constituent flavonoids were structurally characterized using UPLC-PDA-MS 2 . Antioxidant activity (DPPH), Fe 2+ -induced lipid peroxidation (LP) and anticholinesterase activity (Ellman's method) of the BPFRF and standards (ascorbic acid and rivastigmine) across a concentration range of 3.125-100   μg/mL were evaluated in vitro for 4 months. Molecular docking was performed to give insight into the binding potentials of BPFRF constituents against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE).
Results: UPLC-PDA-MS 2 analysis of BPFRF identified carlinoside, quercetin (most dominant), luteolin, isorhamnetin, luteolin-7-glucoside. Carlinoside was first reported in this plant. BPFRF significantly inhibited DPPH radical (IC 50 = 7.382 ± 0.79 µg/mL) and LP (IC 50 = 7.182 ± 0.60 µg/mL) better than quercetin and ascorbic acid. Also, BPFRF exhibited potent inhibition against AChE and BuChE with IC 50 values of 22.283 ± 0.27 µg/mL and 33.437 ± 1.46 µg/mL, respectively compared to quercetin and rivastigmine. Docking studies revealed that luteolin-7-glucoside, carlinoside and quercetin interact effectively with crucial amino acid residues of AChE and BuChE through hydrogen bonds.
Discussion and Conclusions: BPFRF possesses an excellent natural source of cholinesterase inhibitor and antioxidant. The material could be further explored for the potential treatment of oxidative damage and cholinergic dysfunction in Alzheimer's disease.
Databáze: MEDLINE
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