Hydrogel containing (1 → 6)-β-D-glucan (lasiodiplodan) effectively promotes dermal wound healing.

Autor: Nissola C; Programa de Pós-Graduação em Biotecnologia, Universidade Tecnológica Federal do Paraná, Câmpus Dois Vizinhos, CEP 85660-000 Dois Vizinhos, Paraná, Brazil., Marchioro MLK; Departamento de Química, Universidade Tecnológica Federal do Paraná, Campus Pato Branco, CEP 85503-390 Pato Branco, Paraná, Brazil., de Souza Leite Mello EV; Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, CEP 87020-900 Maringá, Paraná, Brazil., Guidi AC; Departamento de Farmácia, Laboratório de Biologia Farmacêutica, Universidade Estadual de Maringá, CEP 87020-900 Maringá, Paraná, Brazil., de Medeiros DC; Departamento de Farmácia, Laboratório de Biologia Farmacêutica, Universidade Estadual de Maringá, CEP 87020-900 Maringá, Paraná, Brazil., da Silva CG; Departamento de Farmácia, Laboratório de Biologia Farmacêutica, Universidade Estadual de Maringá, CEP 87020-900 Maringá, Paraná, Brazil., de Mello JCP; Departamento de Farmácia, Laboratório de Biologia Farmacêutica, Universidade Estadual de Maringá, CEP 87020-900 Maringá, Paraná, Brazil., Pereira EA; Departamento de Química, Universidade Tecnológica Federal do Paraná, Campus Pato Branco, CEP 85503-390 Pato Branco, Paraná, Brazil., Barbosa-Dekker AM; Departamento de Química, Centro de Ciências Exatas, Universidade Estadual de Londrina, CEP 86057-970 Londrina, Paraná, Brazil., Dekker RFH; Programa de Pós-Graduação em Engenharia Ambiental, Universidade Tecnológica Federal do Paraná, Câmpus Londrina, CEP 86036-370 Londrina, Paraná, Brazil., Cunha MAA; Programa de Pós-Graduação em Biotecnologia, Universidade Tecnológica Federal do Paraná, Câmpus Dois Vizinhos, CEP 85660-000 Dois Vizinhos, Paraná, Brazil; Departamento de Química, Universidade Tecnológica Federal do Paraná, Campus Pato Branco, CEP 85503-390 Pato Branco, Paraná, Brazil. Electronic address: mcunha@utfpr.edu.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2021 Jul 31; Vol. 183, pp. 316-330. Date of Electronic Publication: 2021 Apr 27.
DOI: 10.1016/j.ijbiomac.2021.04.169
Abstrakt: A hydrogel containing exocellular (1 → 6)-β-D-glucan (lasiodiplodan, LAS) was developed and its wound healing potential was evaluated. β-Glucans have attracted much interest by the cosmetic industry sector because of their bioactive and functional properties and in promoting skin health. In the present work an β-glucan was studied as a healing biomaterial that has not hitherto been reported in the scientific literature. LAS produced by the ascomycete Lasiodiplodia theobromae MMPI was used in the formulation of a healing hydrogel. Physicochemical and microbiological quality parameters, antioxidant potential and stability of the formulation was evaluated. FTIR, thermal analysis and SEM techniques were also employed in the characterization. Wistar rats were used as a biological model to investigate the wound healing potential. Histological analyses of cutaneous tissue from the dorsal region were conducted after 4, 7, 10 and 14 days of treatment, and evaluated re-epithelialization, cell proliferation and collagen production. Physicochemical stability, microbiological quality and antioxidant potential, especially in relation to its ability to scavenge hydroxyl radicals were found. The hydrogel stimulated cell re-epithelialization and proliferation during all days of the treatment, and stimulated an increase of collagen fibers. Lasiodiplodan showed immunomodulatory activity in wound healing and this biomacromolecule could be an alternative compound in wound care.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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