Sigma factor dependent translational activation in Bacillus subtilis .
| Autor: | McCormick DM; Massachusetts Institute of Technology., Lalanne JB; University of Washington., Lan TCT; Whitehead Institute for Biomedical Research., Rouskin S; Whitehead Institute for Biomedical Research., Li GW; Massachusetts Institute of Technology; gwli@mit.edu. |
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| Jazyk: | angličtina |
| Zdroj: | RNA (New York, N.Y.) [RNA] 2021 Apr 29. Date of Electronic Publication: 2021 Apr 29. |
| DOI: | 10.1261/rna.078747.121 |
| Abstrakt: | Sigma factors are an important class of bacterial transcription factors that lend specificity to RNA polymerases by binding to distinct promoter elements for genes in their regulons. Here we show that activation of the general stress sigma factor, σ B , in Bacillus subtilis paradoxically leads to dramatic induction of translation for a subset of its regulon genes. These genes are translationally repressed when transcribed by the housekeeping sigma factor, σ A , owing to extended RNA secondary structures as determined in vivo using DMS-MaPseq. Transcription from σ B -dependent promoters ablates the secondary structures and activates translation, leading to dual induction. Translation efficiencies between σ B - and σ A -dependent RNA isoforms can vary by up to 100-fold, which in multiple cases exceeds the magnitude of transcriptional induction. These results highlight the role of long-range RNA folding in modulating translation and demonstrate that a transcription factor can regulate protein synthesis beyond its effects on transcript levels. (Published by Cold Spring Harbor Laboratory Press for the RNA Society.) |
| Databáze: | MEDLINE |
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