| Autor: |
Elmonir W; Department of Hygiene and Preventive Medicine (Zoonoses), Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt., Abd El-Aziz NK; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Tartor YH; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Moustafa SM; Department of Zoonoses, Faculty of Veterinary Medicine, Benha University, Benha 13518, Egypt., Abo Remela EM; Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.; Department of Biology, College of Science, Taibah University, Madina 42353, Saudi Arabia., Eissa R; Department of Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta 31527, Egypt., Saad HA; Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Tawab AA; Department of Microbiology, Faculty of Medicine, Al Azhar University, Cairo 11884, Egypt. |
| Abstrakt: |
This study investigated the frequency of carbapenem and colistin resistance in ESBL-producing K. pneumoniae (ESBLK) isolates recovered from chickens and their environment, contact farm workers and hospitalized patients in Egypt. Further, the phenotypic and genotypic relationships between the community and hospital-acquired K. pneumoniae isolates in the same geographical area were investigated. From 272 total samples, 37 (13.6%) K. pneumoniae isolates were identified, of which 20 (54.1%) were hypervirulent. All isolates (100%) were multidrug-resistant (MDR) with multiple antibiotic resistance (MAR) indices ranging from 0.19 to 0.94. Colistin-resistant isolates (18.9%) displayed colistin MIC values >2 μg/mL, all harbored the mcr -1 gene. All isolates from patients (13/90, 14.4%), workers (5/22, 22.7%), chickens (9/100, 9%) and the environment (10/60, 16.7%) harbored a single or multiple β-lactamase genes, bla SHV , bla TEM , bla CTX-M1 and bla OXA-1 , often in combination with carbapenemase genes ( bla VIM , bla NDM-1 or bla IMP ; 45.9%), the mcr -1 gene (18.9%) or both (13.5%). Enterobacterial repetitive intergenic consensus (ERIC)-PCR genotyping revealed 24 distinct ERIC types (ETs) with a discrimination index of 0.961. Six ETs showed clusters of identical isolates from chicken and human sources. The increased frequency and genetic relatedness of ESBLK and carbapenemase-producing K. pneumoniae (CPK) from chickens and humans pose a public health threat that urge more prudent use of antimicrobials in chicken farms to avoid the propagation and expansion of both ESBLK and CPK from the chicken sources to humans. |
