Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis.

Autor: Ulag S; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey.; Metallurgical and Materials Engineering, Institute of Pure and Applied Sciences, Marmara University, 34722 Istanbul, Turkey., Ilhan E; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey.; Department of Bioengineering, Institute of Pure and Applied Sciences, Marmara University, 34722 Istanbul, Turkey., Demirhan R; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey., Sahin A; Department of Biochemistry, Faculty of Medicine, Marmara University, 34718 Istanbul, Turkey.; Genetic and Metabolic Diseases Research and Investigation Center, Marmara University, 34722 Istanbul, Turkey., Yilmaz BK; Department of Biochemistry, Faculty of Medicine, Marmara University, 34718 Istanbul, Turkey.; Genetic and Metabolic Diseases Research and Investigation Center, Marmara University, 34722 Istanbul, Turkey., Aksu B; Department of Medical Microbiology, Marmara University School of Medicine, 34854 Istanbul, Turkey., Sengor M; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey.; Metallurgical and Materials Engineering Faculty of Technology, Marmara University, 34722 Istanbul, Turkey., Ficai D; Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 060042 Bucharest, Romania.; National Centre for Micro- and Nanomaterials, University Politehnica of Bucharest, 060042 Bucharest, Romania., Titu AM; Industrial Engineering and Management Department, Faculty of Engineering, Lucian Blaga University of Sibiu, 550025 Sibiu, Romania.; Academy of Romanian Scientists, 050094 Bucharest, Romania., Ficai A; Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 060042 Bucharest, Romania.; National Centre for Micro- and Nanomaterials, University Politehnica of Bucharest, 060042 Bucharest, Romania.; Academy of Romanian Scientists, 050094 Bucharest, Romania., Gunduz O; Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, 34722 Istanbul, Turkey.; Metallurgical and Materials Engineering Faculty of Technology, Marmara University, 34722 Istanbul, Turkey.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2021 Apr 28; Vol. 26 (9). Date of Electronic Publication: 2021 Apr 28.
DOI: 10.3390/molecules26092577
Abstrakt: In this research, polyvinyl-alcohol (PVA)/gelatin (GEL)/propolis (Ps) biocompatible nanofiber patches were fabricated via electrospinning technique. The controlled release of Propolis, surface wettability behaviors, antimicrobial activities against the S. aureus and P. aeruginosa , and biocompatibility properties with the mesenchymal stem cells (MSCs) were investigated in detail. By adding 0.5, 1, and 3 wt.% GEL into the 13 wt.% PVA, the morphological and mechanical results suggested that 13 wt.% PVA/0.5 wt.% GEL patch can be an ideal matrix for 3 and 5 wt.% propolis addition. Morphological results revealed that the diameters of the electrospun nanofiber patches were increased with GEL (from 290 nm to 400 nm) and Ps addition and crosslinking process cause the formation of thicker nanofibers. The tensile strength and elongation at break enhancement were also determined for 13 wt.% PVA/0.5 wt.% GEL/3 wt.% Ps patch. Propolis was released quickly in the first hour and arrived at a plateau. Cell culture and contact angle results confirmed that the 3 wt.% addition of propolis reinforced mesenchymal stem cell proliferation and wettability properties of the patches. The antimicrobial activity demonstrated that propolis loaded patches had antibacterial activity against the S. aureus , but for P. aeruginosa , more studies should be performed.
Databáze: MEDLINE
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