| Autor: |
Abdel-Mageed WM; Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.; Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt., Al-Wahaibi LH; Department of Chemistry, Science College, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia., Lehri B; School of Life Sciences Pharmacy and Chemistry, Faculty of Science, Engineering and Computing, Kingston University London, Penrhyn Road, Kingston upon Thames KT1 2EE, UK., Al-Saleem MSM; Department of Chemistry, Science College, Princess Nourah Bint Abdulrahman University, Riyadh 11671, Saudi Arabia., Goodfellow M; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK., Kusuma AB; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.; Indonesian Centre for Extremophile Bioresources and Biotechnology (ICEBB), Faculty of Biotechnology, Sumbawa University of Technology, Sumbawa Besar 84371, Indonesia., Nouioui I; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.; Leibniz-Institut DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany., Soleh H; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK., Pathom-Aree W; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK., Jaspars M; Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Old Aberdeen AB24 3UE, UK., Karlyshev AV; School of Life Sciences Pharmacy and Chemistry, Faculty of Science, Engineering and Computing, Kingston University London, Penrhyn Road, Kingston upon Thames KT1 2EE, UK. |
| Abstrakt: |
A Micromonospora strain, isolate MT25 T , was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n -acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1 H- 15 N HSQC and 1 H- 15 N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea , a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142 T . The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25 T (NCIMB 15245 T , TISTR 2834 T ) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25 T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments. |
