| Autor: |
Segabinazzi LGTM; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, 1008 W Hazelwood Drive, Urbana, IL 61802, USA.; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Sao Paulo 18618681, Brazil.; Ross University School of Veterinary Medicine, Basseterre PO Box 334, St. Kitts, West Indies., Canisso IF; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, 1008 W Hazelwood Drive, Urbana, IL 61802, USA., Podico G; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, 1008 W Hazelwood Drive, Urbana, IL 61802, USA., Cunha LL; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, 1008 W Hazelwood Drive, Urbana, IL 61802, USA., Novello G; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, 1008 W Hazelwood Drive, Urbana, IL 61802, USA.; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Sao Paulo 18618681, Brazil., Rosser MF; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois Urbana Champaign, Urbana, IL 61802, USA., Loux SC; Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40503, USA., Lima FS; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana Champaign, 1008 W Hazelwood Drive, Urbana, IL 61802, USA.; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA., Alvarenga MA; Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Sao Paulo 18618681, Brazil. |
| Abstrakt: |
Microorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or -poor (PPP) plasma. Mares ( n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre- (-48 and -24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Ilβ, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey's post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property. |
