Molecular and Serological Detection of Piroplasms in Horses from Nigeria.

Autor: Idoko IS; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Abuja, Gwagwalada 902101, Nigeria., Edeh RE; Department of Veterinary Medicine, Surgery and Radiology, Faculty of Veterinary Medicine, University of Jos, Jos 930222, Nigeria., Adamu AM; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Abuja, Gwagwalada 902101, Nigeria., Machunga-Mambula S; Department of Microbiology, Faculty of Sciences, University of Abuja, Gwagwalada 902101, Nigeria., Okubanjo OO; Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria 810107, Nigeria., Balogun EO; Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria 810107, Nigeria., Adamu S; Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria 810107, Nigeria., Johnson W; Animal Disease Research Unit, USDA-ARS, Pullman, WA 99164-6630, USA., Kappmeyer L; Animal Disease Research Unit, USDA-ARS, Pullman, WA 99164-6630, USA., Mousel M; Animal Disease Research Unit, USDA-ARS, Pullman, WA 99164-6630, USA., Ueti MW; Animal Disease Research Unit, USDA-ARS, Pullman, WA 99164-6630, USA.; Department of Veterinary Microbiology & Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA.
Jazyk: angličtina
Zdroj: Pathogens (Basel, Switzerland) [Pathogens] 2021 Apr 23; Vol. 10 (5). Date of Electronic Publication: 2021 Apr 23.
DOI: 10.3390/pathogens10050508
Abstrakt: Equine piroplasmosis, an economically important disease of equids caused by the hemoprotozoan parasites Theileria equi , T. haneyi , and Babesia caballi , has a worldwide distribution. These parasites are transmitted by ixodid ticks. To improve the detection of horses in Nigeria exposed to piroplasm parasites, 72 horses with variable clinical signs of piroplasmosis were sampled from Northwest and Northcentral Nigeria and tested by nPCR and cELISA. Blood and serum samples were collected from each horse via jugular venesection. Individually, nPCR or cELISA failed to identify all horses exposed to piroplasms. A combination of species-specific nPCR and the OIE-approved T. equi and B. caballi cELISAs enhanced the detection of horses exposed to parasites. The results also demonstrated horses showing abnormal hematology were positive for only T. equi, except for one sample that was coinfected with T. equi and T. haneyi . We also identified ticks collected from some of the horses, with Rhipicephalus evertsi evertsi being the most prevalent. This study shows that a larger proportion of horses in the sample set were exposed to T. equi than B. caballi or T. haneyi . Additionally, ticks that have been previously reported as potential vectors for these parasites were found to have infested sampled horses. Further studies are needed to investigate which tick species are competent vectors for Theileria spp. and Babesia caballi in Nigeria.
Databáze: MEDLINE