| Autor: |
Elsayed MSAE; Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minufiya 32897, Egypt., Eldsouky SM; Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, Benha University, Benha City, Qalyubia 13511, Egypt., Roshdy T; Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Minufiya 32897, Egypt., Said L; Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minufiya 32897, Egypt., Thabet N; Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minufiya 32897, Egypt., Allam T; Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minufiya 32897, Egypt., Mohammed ABA; Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Minufiya 32897, Egypt., Nasr GM; Department of Molecular Diagnostics, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Minufiya 32897, Egypt., Basiouny MSM; Faculty of Biotechnology, Badr University, Badr City, Cairo 19592, Egypt., Akl BA; Microbiology Department, Faculty of Agriculture, Zagazig University, Zagazig 44519, Egypt., Nader MM; Microbiology Department, Faculty of Agriculture, Zagazig University, Zagazig 44519, Egypt., Hasan AS; Department of Medical Pharmacology, Faculty of Medicine, South Valley University, Qena 83523, Egypt., Salah A; Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Minufiya 32897, Egypt. |
| Abstrakt: |
Pasteurella multocida is a Gram-negative bacterium that causes drastic infections in cattle and humans. In this study, 55 isolates were recovered from 115 nasal swabs from apparently healthy and diseased cattle and humans in Minufiya and Qalyubia, Egypt. These isolates were confirmed by kmt1 existence, and molecular classification of the capsular types showed that types B, D, and E represented 23/55 (41.8%), 21/55 (38.1%), and 11/55 (20.0%), respectively. The isolates were screened for five virulence genes with hgbA , hgbB , and ptfA detected in 28/55 (50.9%), 30/55 (54.5%), and 25/55 (45.5%), respectively. We detected 17 capsular and virulence gene combinations with a discriminatory power (DI) of 0.9286; the most prevalent profiles were dcbF type D and dcbF type D, hgbA , hgbB , and ptfA , which represented 8/55 (14.5%) each. These strains exhibited high ranges of multiple antimicrobial resistance indices; the lowest resistances were against chloramphenicol, ciprofloxacin, amoxicillin/clavulanic acid, and levofloxacin. The macrolide-lincosamide-streptogramin B methylase gene erm (Q), with erm (42) encoding MLS B monomethyltransferase, mph (E) encoding a macrolide efflux pump, and msr (E) encoding macrolide-inactivating phosphotransferase were present. The class 1 and 2 integrons and extended-spectrum β-lactamase genes intl1 , intl2 , bla CTX-M , bla CTX-M-1 , and bla TEM were detected. It is obvious to state that co-occurrence of resistance genes resulted in multiple drug-resistant phenotypes. The identified isolates were virulent, genetically diverse, and resistant to antimicrobials, highlighting the potential risk to livestock and humans. |
