| Autor: |
Ignacio JCI; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines.; University of the Philippines, Los Baños, Laguna 4031, Philippines.; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.; Department of Horticulture and Crop Science, Ohio Agriculture Research and Development Center, The Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA., Zaidem M; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.; Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, UK., Casal C Jr; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines., Dixit S; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines., Kretzschmar T; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines.; Southern Cross Plant Sciences, Southern Cross University, 1 Military Road, Lismore, NSW 2480, Australia., Samaniego JM; University of the Philippines, Los Baños, Laguna 4031, Philippines., Mendioro MS; University of the Philippines, Los Baños, Laguna 4031, Philippines., Weigel D; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany., Septiningsih EM; International Rice Research Institute, DAPO Box 7777, Metro Manila 1301, Philippines.; Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA. |
| Abstrakt: |
Direct seeded rice (DSR) is a mainstay for planting rice in the Americas, and it is rapidly becoming more popular in Asia. It is essential to develop rice varieties that are suitable for this type of production system. ASD1, a landrace from India, possesses several traits desirable for direct-seeded fields, including tolerance to anaerobic germination (AG). To map the genetic basis of its tolerance, we examined a population of 200 F 2:3 families derived from a cross between IR64 and ASD1 using the restriction site-associated DNA sequencing (RAD-seq) technology. This genotyping platform enabled the identification of 1921 single nucleotide polymorphism (SNP) markers to construct a high-resolution genetic linkage map with an average interval of 0.9 cM. Two significant quantitative trait loci (QTLs) were detected on chromosomes 7 and 9, qAG7 and qAG9 , with LOD scores of 7.1 and 15.0 and R 2 values of 15.1 and 29.4, respectively. Here, we obtained more precise locations of the QTLs than traditional simple sequence repeat and low-density SNP genotyping methods and may help further dissect the genetic factors of these QTLs. |
