Disaccahrides-Based Cryo-Formulant Effect on Modulating Phospho/Mitochondrial Lipids and Biological Profiles of Human Leukaemia Cells.

Autor:	Straka MF; Department of Chemical Engineering & Biotechnology, University of Cambridge, Cambridge, UK., Al-Otaibi NA; Department of Chemical Engineering & Biotechnology, University of Cambridge, Cambridge, UK.; King Abdulaziz City for Science and Technology Kingdom of Saudi Arabia, Riyadh, Saudi Arabia., Whitfield PD; Lipidomics Research Facility, University of the Highlands and Islands, Inverness, UK., Doherty MK; Lipidomics Research Facility, University of the Highlands and Islands, Inverness, UK., Matarèse BFE; Department of Chemical Engineering & Biotechnology, University of Cambridge, Cambridge, UK.; Department of Physics, University of Cambridge, Cavendish Laboratory, Cambridge, UK.; Department of Haematology, University of Cambridge, Cavendish Laboratory, Cambridge, UK., Slater NKH; Department of Chemical Engineering & Biotechnology, University of Cambridge, Cambridge, UK., Rahmoune H; Department of Chemical Engineering & Biotechnology, University of Cambridge, Cambridge, UK, hr288@cam.ac.uk.
Jazyk:	angličtina
Zdroj:	Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2021 Apr 30; Vol. 55 (2), pp. 206-221.
DOI:	10.33594/000000360
Abstrakt:	Background/aims: The use of novel cryo-additive agents to increase cell viability post-cryopreservation is paramount to improve future cell based-therapy treatments. We aimed to establish the Human Leukemia (HL-60) cells lipidomic and biological patterns when cryo-preserved in DMSO alone and with 300 µM Nigerose (Nig), 200 µM Salidroside (Sal) or a combination of Nig (150 µM) and Sal (100 µM). Methods: HL-60 cells were pre-incubated with Nig/Sal prior, during and post cryopreservation, and subjected to global lipidomic analysis. Malondialdeyhde (MDA), released lactate dehydrogenase (LDH) and reactive oxygen scavenger (ROS) measurements were also carried out to evaluate levels of lipid peroxidation and cytotoxicity. Results: Cryopreserving HL-60 cells in DMSO with Nig and Sal provided optimal protection against unsaturated fatty acid oxidation. Post-thaw, cellular phospholipids and mitochondrial cardiolipins were increased by Nig/Sal as the ratio of unsaturated to saturated fatty acids 2.08 +/- 0.03 and 0.95 +/- 0.09 folds respectively in comparison to cells cryopreserved in DMSO alone (0.49 +/- 0.05 and 0.86 +/- 0.10 folds). HL-60 lipid peroxidation levels in the presence of DMSO + Nig and Sal combined were significantly reduced relative to pre-cryopreservation levels (10.91 +/- 2.13 nmole) compared to DMSO (17.1 +/- 3.96 nmole). DMSO + Nig/Sal combined also significantly reduced cell cytotoxicity post-thaw (0.0128 +/- 0.00182 mU/mL) in comparison to DMSO (0.0164 +/- 0.00126 mU/mL). The combination of Nig/Sal also reduced significantly ROS levels to the levels of prior cryopreservation of HL-60. Conclusion: Overall, the establishment of the cryopreserved HL-60 cells lipidomic and the corresponding biological profiles showed an improved cryo-formulation in the presence of DMSO with the Nig/Sal combination by protecting the, mitochondrial inner membrane, unsaturated fatty acid components (i. e. Cardiolipins) and total phospholipids. Competing Interests: The authors have no conflicts of interest to declare. (© Copyright by the Author(s). Published by Cell Physiol Biochem Press.)
Databáze:	MEDLINE
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