Gluconeogenesis is reduced from alanine, lactate and pyruvate, but maintained from glycerol, in liver perfusion of rats with early and late sepsis.

Autor: de Souza Galia WB; Department of Physiological Sciences, State University of Londrina, Londrina, Brazil., Biazi GR; Department of Physiological Sciences, State University of Londrina, Londrina, Brazil., Frasson-Uemura IG; Department of Physiological Sciences, State University of Londrina, Londrina, Brazil., Miksza DR; Department of Physiological Sciences, State University of Londrina, Londrina, Brazil., Zaia CTBV; Department of Physiological Sciences, State University of Londrina, Londrina, Brazil., Zaia DAM; Department of Chemistry, State University of Londrina, Londrina, Brazil., de Souza HM; Department of Physiological Sciences, State University of Londrina, Londrina, Brazil., Bertolini GL; Department of Physiological Sciences, State University of Londrina, Londrina, Brazil.
Jazyk: angličtina
Zdroj: Cell biochemistry and function [Cell Biochem Funct] 2021 Aug; Vol. 39 (6), pp. 754-762. Date of Electronic Publication: 2021 Apr 29.
DOI: 10.1002/cbf.3637
Abstrakt: Sepsis induces several metabolic abnormalities, including hypoglycaemia in the most advanced stage of the disease, a risk factor for complications and death. Although hypoglycaemia can be caused by inhibition of hepatic gluconeogenesis, decreased and increased gluconeogenesis were reported in sepsis. Furthermore, gluconeogenesis from glycerol was not yet evaluated in this disease. The main purpose of this study was to investigate the gluconeogenesis from alanine, lactate, pyruvate and glycerol in rats with early (8 hours) and late (18 hours) sepsis. Parameters related to the characterization of sepsis were also evaluated. Sepsis was induced by cecal ligation and puncture and gluconeogenesis was assessed in liver perfusion. Rats with early and late sepsis showed increased lactataemia, depletion of liver glycogen and peripheral insulin resistance, characterizing the establishment of sepsis. Rats with early and late sepsis showed decreased gluconeogenesis from alanine, lactate and pyruvate. Interestingly, gluconeogenesis from glycerol, a precursor that enters in the pathway at a later step, subsequent to the entry of alanine, lactate and pyruvate, was maintained in rats with early and late sepsis. In conclusion, gluconeogenesis is decreased from alanine, lactate and pyruvate, but maintained from glycerol, in liver perfusion of rats with early and late sepsis. SIGNIFICANCE OF THE STUDY: The maintenance of gluconeogenesis from glycerol, but not from alanine, lactate and pyruvate, together with the liver glycogen depletion, points the glycerol as an important precursor for the maintenance of glycaemic homeostasis in sepsis. The findings open the possibility of further investigation on the administration of glycerol in the treatment of hypoglycaemia associated with more advanced sepsis.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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