Detecting single cell interferon-beta production using a fluorescent reporter telomerase-immortalized human fibroblast cell line.
| Autor: | Hare DN; Health Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada., Subapanditha MK; Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada., Mossman KL; Health Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.; Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2021 Apr 10; Vol. 2 (2), pp. 100436. Date of Electronic Publication: 2021 Apr 10 (Print Publication: 2021). |
| DOI: | 10.1016/j.xpro.2021.100436 |
| Abstrakt: | Recent data suggest that cells respond to infection by upregulating the antiviral cytokine interferon-beta (IFN-ß) in a fraction of infected cells. Approaches are thus needed to study these responses on a single-cell level rather than bulk population. Here, we describe a protocol to analyze the IFN-ß response of individual cells using flow cytometry and immunofluorescence microscopy. We show the heterogeneous IFN-ß response to inactivated Sendai virus and human cytomegalovirus, but this protocol can be adapted to other viruses. For complete details on the use and execution of this protocol, please refer to Hare et al. (2020). Competing Interests: The authors declare no competing interest. (© 2021 The Author(s).) |
| Databáze: | MEDLINE |
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