Platelet-derived growth factor-B signalling might promote epithelial-mesenchymal transition in gastric carcinoma cells through activation of the MAPK/ERK pathway.
| Autor: | Yin J; Department of Ultrasound, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China., Guo Y; Department of General Surgery, Chongqing University Central Hospital (Chongqing Emergency Medical Center), Chongqing, China., Li Z; Department of General Surgery, Chongqing University Central Hospital (Chongqing Emergency Medical Center), Chongqing, China. |
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| Jazyk: | angličtina |
| Zdroj: | Contemporary oncology (Poznan, Poland) [Contemp Oncol (Pozn)] 2021; Vol. 25 (1), pp. 1-6. Date of Electronic Publication: 2021 Mar 22. |
| DOI: | 10.5114/wo.2021.103938 |
| Abstrakt: | Introduction: Epithelial-mesenchymal transition (EMT) is important in the metastasis of tumours and is triggered by several key growth factors, including platelet-derived growth factor-B (PDGF-B). But, whether PDGF-B signalling promotes EMT in gastric carcinoma cells is still unknown. Material and Methods: We established 2 gastric carcinoma cell lines (MKN28 and MKN45) to stably overexpress PDGF-B by lentiviral vectors, and expression of E-cadherin, N-cadherin, and ERK-1 were detected by western blot assay. Then, PDGF-B overexpression and normal MKN28 and MKN45 cells were cocultured with PDGFR-b positive fibroblast (hs738) and MAPK inhibitors were added; also, the expressions of ERK-1, E-cadherin, and N-cadherin were detected by western blot assay. Results: After being cocultured with hs738 cells, expressions of ERK-1 and N-cadherin protein in PDGF-B overexpression MKN28 and MKN45 cells were much higher than normal MKN28 and MKN45 cells ( p < 0.05), and those could be decreased by MAPK inhibitor. Also, expressions of E-cadherin protein in PDGF-B overexpression MKN28 and MKN45 cells were much lower than normal MKN28 and MKN45 cells ( p < 0.05), and they could be increased by MAPK inhibitor. Conclusions: Our data indicate that PDGF-B signalling can induce EMT in gastric carcinoma cells. Thr tumour microenvironment is imperative in the process of PDGF-B signalling inducing EMT in gastric carcinoma cells. Also, activation of MAPK/ERK pathway, which is a downstream pathway of PDGF-B signalling, might participate in this process. Competing Interests: The authors declare no conflict of interest. (Copyright © 2021 Termedia.) |
| Databáze: | MEDLINE |
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