Cymbopogon citratus (DC.) Stapf, citral and geraniol exhibit anticonvulsant and neuroprotective effects in pentylenetetrazole-induced seizures in zebrafish.

Autor: Hacke ACM; Departamento de Química, Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, Brazil. Electronic address: ana_hacke@hotmail.com., Miyoshi E; Departamento de Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, Brazil. Electronic address: edmiyoshi@gmail.com., Marques JA; Departamento de Química, Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, Brazil. Electronic address: amarques.jacqueline@gmail.com., Pereira RP; Departamento de Química, Universidade Estadual de Ponta Grossa, UEPG, Ponta Grossa, Brazil. Electronic address: romaiana@uepg.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2021 Jul 15; Vol. 275, pp. 114142. Date of Electronic Publication: 2021 Apr 25.
DOI: 10.1016/j.jep.2021.114142
Abstrakt: Ethnopharmacological Relevance: Cymbopogon citratus (DC.) Stapf (C. citratus) is consumed as an infusion in folk medicine due to its pharmacological properties and action in the central nervous system. Epilepsy is a neurological disorder that affects millions of people. Since the currently available antiepileptic drugs often cause undesirable side effects, new alternative therapeutic strategies based on medicinal plants have been proposed.
Aim of the Study: This study aimed to investigate the anticonvulsant and neuroprotective effects of C. citratus essential oil (EO) and hydroalcoholic extract (E1) from its leaves, as well as of its related compounds citral (CIT) and geraniol (GER) against the effects of pentylenetetrazole (PTZ) induced seizures in zebrafish (Danio rerio).
Materials and Methods: To evaluate the anticonvulsant properties of the samples, adult animals were pre-treated (by immersion) and subsequently exposed to PTZ solution. The involvement of GABA A receptors in the antiepileptic effects was investigated by the coadministration of flumazenil (FMZ), a known GABA A receptor antagonist. Oxidative stress markers malondialdehyde (MDA), glutathione (GSH), catalase (CAT) and nitric oxide (NO) were assessed in zebrafish brain homogenates after PTZ exposure.
Results: All samples increased the latency time for the first seizure, which was reduced when animals were pretreated with FMZ, suggesting the involvement of GABA A receptors in the observed properties. The association between CIT and GER at the lowest concentration studied showed a synergistic effect on the anticonvulsant activity. Decreases in MDA and NO levels and increases in GSH and CAT levels in the brain of treated animals suggested the neuroprotective effect of the compounds investigated.
Conclusions: Our results proved that C. citratus EO, E1, CIT and GER have anticonvulsant effects in zebrafish and could be used as a promising adjuvant therapeutic strategy for epilepsy treatment. Furthermore, zebrafish demonstrated to be an alternative animal model of epilepsy to evaluate the anticonvulsant and neuroprotective effects of C. citratus.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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