Sensitization with Eustrongylides sp. (Nematoda: Dioctophymatidae) antigens induce production of specific IgG and IgE in murine model.

Autor:	Kuraiem BP; Laboratório de Inspeção e Tecnologia do Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil., Verícimo MA; Laboratório de Imunobiologia das Doenças Infecciosas e Granulomatosas, Instituto de Biologia, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil., Knoff M; Laboratório de Helmintos Parasitos de Vertebrados, Instituto Oswaldo Cruz, Fundação Oswaldo CRUZ - FIOCRUZ, Rio de Janeiro, RJ, Brasil., Mattos DPBG; Laboratório de Parasitologia, Instituto Biomédico, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil., São Clemente SC; Laboratório de Inspeção e Tecnologia do Pescado, Faculdade de Medicina Veterinária, Universidade Federal Fluminense - UFF, Niterói, RJ, Brasil.
Jazyk:	angličtina
Zdroj:	Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria [Rev Bras Parasitol Vet] 2021 Apr 26; Vol. 30 (1), pp. e023920. Date of Electronic Publication: 2021 Apr 26 (Print Publication: 2021).
DOI:	10.1590/S1984-29612021018
Abstrakt:	Fish consumption plays an important role in human diet. Hoplias malabaricus, commonly known as traíra, is a freshwater fish widely appreciated in several Brazilian states and frequently infected by Eustrongylides sp. fourth-instar larvae (L4). The aim of the present study was to evaluate allergenic potential of Eustrongylides sp. L4 crude extract (CEE). BALB/c mice were immunized intraperitoneally (IP) by 10 μg CEE with 2 mg of aluminum hydroxide on days 0 and 35. Specific IgG and IgE antibody levels were determined after immunization and cellular immunity was evaluated by assessing intradermal reaction in ear pavilion. Epicutaneous sensitization was performed in dorsal region by antigen exposure using a Finn-type chamber containing 50 μg of CEE or saline solution, followed by evaluation of specific antibody levels. IP immunization resulted in a gradual increase in IgG antibody levels and transitory IgE production. Significant increase in ear thickness was observed in cellular hypersensitivity reaction. In case of antigen exposure by epicutaneous route, CEE was able to induce meaningfully increased levels of specific IgG and IgE antibodies as well as heightened cellular immunity. Both intraperitoneal immunization and epicutaneous contact with Eustrongylides sp. larval antigens were observed for first time to be capable of inducing immunological sensitization in mice.
Databáze:	MEDLINE
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