| Autor: |
Tufa TB; College of Health Science, Arsi University, Asella, Ethiopia. tafeseb.tufa@yahoo.com.; Hirsch Institute of Tropical Medicine, Asella, Ethiopia. tafeseb.tufa@yahoo.com.; Department of Gastroenterology, Hepatology and Infectious Diseases, Heinrich Heine University, Düsseldorf, Germany. tafeseb.tufa@yahoo.com., Wölfel S; Bundeswehr Institute of Microbiology, Munich, Germany.; Amedes MVZ for Laboratory Medicine and Microbiology, Fürstenfeldbruck, Germany., Zubriková D; Institute of Parasitology, Slovak Academy of Sciences, Kosice, Slovakia., Víchová B; Institute of Parasitology, Slovak Academy of Sciences, Kosice, Slovakia., Andersson M; Arlöv, Malmö, Sweden., Rieß R; Bundeswehr Institute of Microbiology, Munich, Germany., Rutaihwa L; Swiss Tropical and Public Health Institute, Basel, Switzerland., Fuchs A; Hirsch Institute of Tropical Medicine, Asella, Ethiopia.; Department of Gastroenterology, Hepatology and Infectious Diseases, Heinrich Heine University, Düsseldorf, Germany., Orth HM; Hirsch Institute of Tropical Medicine, Asella, Ethiopia.; Department of Gastroenterology, Hepatology and Infectious Diseases, Heinrich Heine University, Düsseldorf, Germany., Häussinger D; Hirsch Institute of Tropical Medicine, Asella, Ethiopia.; Department of Gastroenterology, Hepatology and Infectious Diseases, Heinrich Heine University, Düsseldorf, Germany., Feldt T; Hirsch Institute of Tropical Medicine, Asella, Ethiopia.; Department of Gastroenterology, Hepatology and Infectious Diseases, Heinrich Heine University, Düsseldorf, Germany., Poppert S; Swiss Tropical and Public Health Institute, Basel, Switzerland.; University Basel, Basel, Switzerland., Dobler G; Bundeswehr Institute of Microbiology, Munich, Germany., Bakkes DK; Gertrud Theiler Tick Museum, Agricultural Research Council-Onderstepoort Veterinary Research, Pretoria, South Africa.; Evolutionary Genomics Group, Department of Botany and Zoology, Stellenbosch University, Stellenbosch, South Africa., Chitimia-Dobler L; Bundeswehr Institute of Microbiology, Munich, Germany. |
| Abstrakt: |
Ticks will diminish productivity among farm animals and transmit zoonotic diseases. We conducted a study to identify tick species infesting slaughter bulls from Adama City and to screen them for tick-borne pathogens. In 2016, 291 ticks were collected from 37 bulls in Adama, which were ready for slaughter. Ticks were identified morphologically. Total genomic DNA was extracted from ticks and used to test for Rickettsia spp. with real-time PCR. Species identification was done by phylogenetic analysis using sequencing that targeted the 23S-5S intergenic spacer region and ompA genes. Four tick species from two genera, Amblyomma and Rhipicephalus, were identified. Amblyomma cohaerens was the dominant species (n = 241, 82.8%), followed by Amblyomma variegatum (n = 22, 7.5%), Rhipicephalus pulchellus (n = 19, 6.5%), and Rhipicephalus decoloratus (n = 9, 3.0%). Among all ticks, 32 (11%) were positive for Rickettsia spp. and 15 (5.2%) of these were identified as R. africae comprising at least two genetic clades, occurring in A. variegatum (n = 10) and A. cohaerens (n = 5). The remainder of Rickettsia-positive samples could not be amplified due to low DNA yield. Furthermore, another 15 (5.2%) samples carried other pathogenic bacteria: Ehrlichia ruminantium (n = 9; 3.1%) in A. cohaerens, Ehrlichia sp. (n = 3; 1%) in Rh. pulchellus and A. cohaerens, Anaplasma sp. (n = 1; 0.5%) in A. cohaerens, and Neoehrlichia mikurensis (n = 2; 0.7%) in A. cohaerens. All ticks were negative for Bartonella spp., Babesia spp., Theileria spp., and Hepatozoon spp. We reported for the first time E. ruminatium, N. mikurensis, Ehrlichia sp., and Anaplasma sp. in A. cohaerens. Medically and veterinarily important pathogens were mostly detected from A. variegatum and A. cohaerens. These data are relevant for a One-health approach for monitoring and prevention of tick-borne disease transmission. |
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