| Autor: |
Phukuntsi MA; South African National Biodiversity Institute, P.O. Box 754, Pretoria, 0001, South Africa.; Department of Environment, Water and Earth Sciences, Tshwane University of Technology, Private Bag X360, Pretoria, 0001, South Africa., Du Plessis M; South African National Biodiversity Institute, P.O. Box 754, Pretoria, 0001, South Africa.; Department of Biotechnology, University of Western Cape, Robert Sobukwe Road, Bellville, Cape Town, 7535, South Africa., Dalton DL; South African National Biodiversity Institute, P.O. Box 754, Pretoria, 0001, South Africa. d.dalton@sanbi.org.za., Jansen R; Department of Environment, Water and Earth Sciences, Tshwane University of Technology, Private Bag X360, Pretoria, 0001, South Africa., Sauther ML; Department of Anthropology, University of Colorado, Boulder, CO, 80302, USA., Cuozzo FP; Lajuma Research Centre, P.O. Box 522, Louis Trichardt (Makhado), 0920, South Africa., Kotze A; South African National Biodiversity Institute, P.O. Box 754, Pretoria, 0001, South Africa.; Department of Genetics, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa. |
| Abstrakt: |
The habitats of Galago moholi are suspected to be largely fragmented, while the species is thought to be expanding further into the southernmost fringe of its range, as well as into human settlements. To date, no intraspecific molecular genetic studies have been published on G. moholi. Here we estimate the genetic diversity and connectivity of populations of G. moholi using two mitochondrial gene regions, the cytochrome C oxidase subunit I gene (COI) and the displacement loop of the control region (D-loop). Samples from five localities in northern South Africa were obtained from archived collections. The two mitochondrial DNA gene regions were amplified and sequenced to provide population summary statistics, differentiation [proportion of the total genetic variation in a population relative to the total genetic variance of all the populations (F ST ), differentiation within populations among regions (Φ ST )], genetic distance and structure. There was discernible genetic structure among the individuals, with two COI and six D-loop haplotypes belonging to two genetically different groups. There was population differentiation among regions (F ST = 0.670; Φ ST = 0.783; P < 0.01). However, there were low levels of differentiation among populations, as haplotypes were shared between distant populations. Adjacent populations were as divergent from each other as from distant populations. The results suggest that genetic introgression, most likely due to past migrations or recent unintentional translocations that include the animal trade, may have led to connectivity among populations. |
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