Serological evidence of West Nile viral infection in archived swine serum samples from Peninsular Malaysia.

Autor: Mohammed MN; Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.; Department of Animal Production, Federal University of Technology Minna, PMB 65, Minna, Niger, Nigeria., Yasmin AR; Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia.; Laboratory of Vaccines and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia. noryasmin@upm.edu.my., Noraniza MA; Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia., Ramanoon SZ; Department of Farm and Exotic Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia., Arshad SS; Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia., Bande F; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Bayero University, PMB 3011, Kano, Nigeria., Mohammed HO; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Jazyk: angličtina
Zdroj: Journal of veterinary science [J Vet Sci] 2021 May; Vol. 22 (3), pp. e29. Date of Electronic Publication: 2021 Mar 05.
DOI: 10.4142/jvs.2021.22.e29
Abstrakt: West Nile virus (WNV), a neurotropic arbovirus, has been detected in mosquitos, birds, wildlife, horses, and humans in Malaysia, but limited information is available on WNV infection in Malaysian pigs. We tested 80 archived swine serum samples for the presence of WNV antibody and West Nile (WN) viral RNA using ID Screen West Nile Competition Multi-species enzyme-linked immunosorbent assay kits and WNV-specific primers in reverse transcription polymerase chain reaction assays, respectively. A WNV seroprevalence of 62.5% (50/80) at 95% confidence interval (51.6%-72.3%) was recorded, with a significantly higher seroprevalence among young pigs (weaner and grower) and pigs from south Malaysia. One sample was positive for Japanese encephalitis virus antibodies; WN viral RNA was not detected in any of the serum samples.
Competing Interests: The authors declare no conflicts of interest.
(© 2021 The Korean Society of Veterinary Science.)
Databáze: MEDLINE