| Autor: |
Nordgren M; Genovis AB, Lund, Sweden., Nägeli A; Genovis AB, Lund, Sweden., Nyhlén H; Genovis AB, Lund, Sweden., Sjögren J; Genovis AB, Lund, Sweden. jonathan.sjogren@genovis.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2271, pp. 155-167. |
| DOI: |
10.1007/978-1-0716-1241-5_11 |
| Abstrakt: |
O-glycosylation is a difficult posttranslational modification to analyze. O-glycans are labile and often cluster making their analysis by LC-MS very challenging. OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This enables the generation of glycopeptides of suitable size for mapping O-glycosylation sites in detail by bottom-up LC-MS analysis. In this chapter we demonstrate a simple workflow for in-depth analysis of O-glycosylation sites on heavily glycosylated proteins using OpeRATOR digestion and HILIC-MS/MS analysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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