| Autor: |
Hazzaa SM; Medical Physiology, Menoufia University, Shebein Elkom, Egypt., Abdou AG; Pathology Departments, Faculty of Medicine, Menoufia University, Shebein Elkom, Egypt., Ibraheim EO; Medical Physiology, Menoufia University, Shebein Elkom, Egypt., Salem EA; Medical Physiology, Menoufia University, Shebein Elkom, Egypt., Hassan MHA; Medical Physiology, Menoufia University, Shebein Elkom, Egypt., Abdel-Razek HAD; Medical Physiology, Menoufia University, Shebein Elkom, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunoassay & immunochemistry [J Immunoassay Immunochem] 2021 Nov 02; Vol. 42 (6), pp. 596-619. Date of Electronic Publication: 2021 Apr 26. |
| DOI: |
10.1080/15321819.2021.1914085 |
| Abstrakt: |
Pro-inflammatory cytokines and reactive oxygen species (ROS) are produced in acute spinal cord injury, leading to myelin breakdown, inflammation, mitochondrial dysfunction, and apoptosis of neurons and glial cells. The aim of the present study was to investigate possible protective effects of L-carnitine (carn) or atorvastatin (ator) on spinal cord ischemia-reperfusion injury (IRI). Rats were randomized into nine equal groups (n = 8): control and control taking carn (100 mg/kg BW), ator (2.5 mg/kg BW) or both, as well as sham-operation, IRI and IRI taking same doses of carn, ator or both. Neurological assessments were done 48 hours after IRI, and serum nitrite/nitrate was measured. Finally, lumbar segments of spinal cord were excised, and part was homogenized and prepared for measuring tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), malondialdehyde (MDA), advanced oxidation protein products (AOPP), reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase. The other part was sectioned for evaluation of histopathological changes and for immunostaining by glial fibrillary acidic protein (GFAP), Bax and Bcl-2. The IRI increased ROS (nitrite/nitrate, MDA, AOPP) and pro-inflammatory cytokines (TNF-α, IL-1β), and decreased antioxidants (GSH, GPx, SOD, catalase) with impaired sensory and motor functions. Astrogliosis was detected by GFAP, and increased apoptosis was demonstrated by increasing Bax and decreasing Bcl-2. Treatment with carn or ator alone decreased TNF-α, IL-1β, nitrite/nitrate, MDA and AOPP, and increased GSH, GPx, SOD, and catalase with improvement of neurological functions and histological studies. Combination of carn and ator improved most of measured IRI-affected parameters better than isolated carn or ator administration. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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