Complete genome sequence of a grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant from South Africa.

Autor: Bester R; Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa., Lotos L; Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece., Vermeulen A; ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute of the Agricultural Research Council), Stellenbosch, 7599, South Africa., Pietersen G; Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa., Maliogka VI; Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloníki, Greece., Maree HJ; Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa. hjmaree@sun.ac.za.; Citrus Research International, Stellenbosch, 7600, South Africa. hjmaree@sun.ac.za.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2021 Jul; Vol. 166 (7), pp. 2041-2044. Date of Electronic Publication: 2021 Apr 26.
DOI: 10.1007/s00705-021-05079-x
Abstrakt: High-throughput sequencing (HTS) was used to construct the virome profile of an old grapevine-leafroll-diseased grapevine (Vitis vinifera). De novo assembly of HTS data showed a complex infection, including a virus sequence with similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 7090 nucleotides and has four open reading frames (ORFs). Genome organisation and phylogenetic analysis identify this virus as a divergent variant of grapevine Roditis leaf discoloration-associated virus (GRLDaV) with 90% nucleotide sequence identity to isolate w4 (NC_027131). This is the first genome sequence of a South African variant of GRLDaV.
Databáze: MEDLINE
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