Combining whole-cell patch clamp and dye loading in acute brain slices with bulk RNA sequencing in embryonic to aged mice.
| Autor: | Kamen Y; Wellcome - Medical Research Council Cambridge Stem Cell Institute and Department of Veterinary Medicine, University of Cambridge, Cambridge CB2 0AW, United Kingdom., Káradóttir RT; Wellcome - Medical Research Council Cambridge Stem Cell Institute and Department of Veterinary Medicine, University of Cambridge, Cambridge CB2 0AW, United Kingdom.; Department of Physiology, Biomedical Centre, Faculty of Medicine, University of Iceland, Reykjavik, Iceland. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2021 Apr 09; Vol. 2 (2), pp. 100439. Date of Electronic Publication: 2021 Apr 09 (Print Publication: 2021). |
| DOI: | 10.1016/j.xpro.2021.100439 |
| Abstrakt: | Single-cell electrophysiological recordings combined with dye loading and immunohistochemistry provide unparalleled single-cell resolution of cell physiology, morphology, location, and protein expression. When correlated with bulk RNA sequencing, these data can define cell identity and function. Here, we describe a protocol to prepare acute brain slices from embryonic and postnatal mice for whole-cell patch clamp, dye loading and post-hoc immunohistochemistry, and cell isolation for bulk RNA sequencing. While we focus on oligodendrocyte precursor cells, this protocol is applicable to other brain cells. For complete details on the use and execution of this protocol, please refer to Spitzer et al. (2019). Competing Interests: The authors declare no competing interests. (© 2021 The Author(s).) |
| Databáze: | MEDLINE |
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