Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF).

Autor:	Steffanowski C; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, IbaViM, Argentina., Papalia M; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, IbaViM, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina., Iriarte A; Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Montevideo, Uruguay., Langleib M; Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Montevideo, Uruguay., Galanternik L; Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina., Gutkind G; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, IbaViM, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina., Cooper V; University of Pittsburgh, Department of Microbiology and Molecular Genetics, Pittsburgh, United States., Ramírez MS; California State University, Fullerton, Department of Biological Science, Fullerton, United States., Radice M; Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, IbaViM, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: marcelaradice@gmail.com.
Jazyk:	angličtina
Zdroj:	Revista Argentina de microbiologia [Rev Argent Microbiol] 2022 Jan-Mar; Vol. 54 (1), pp. 3-8. Date of Electronic Publication: 2021 Apr 23.
DOI:	10.1016/j.ram.2021.01.005
Abstrakt:	In the last decade Achromobacter spp. has been associated with chronic colonization in patients with cystic fibrosis (CF). Although Achromobacter xylosoxidans is the most frequent species recovered within this genus, other species such as A. ruhlandii have also been reported in these patients. Descriptions of mobile elements are scarce in Achromobacter and none of them have been originated in A. ruhlandii. The aim of this study was to report the full characterization of a plasmid which was maintained in four clonally related A. ruhlandii isolates. Between 2013 and 2015, nine A. ruhlandii isolates were recovered from a pediatric patient with CF at a hospital in Buenos Aires. Four selected clonally related isolates were sequenced by Illumina MiSeq, annotated using RAST and manually curated. The presence of a unique plasmid of 34096-bp and 50 CDS was observed in the four isolates, displaying only 1 nucleotide substitution translated into one amino acid change among them. These plasmids have a class 1 integron containing the aac-(6')-Ib gene, a mercury resistance operon region and the relE/stbE toxin/antitoxin system. Plasmids showed 79% similarity and 99% identity with pmatvim-7 from Pseudomonas aeruginosa. This is the first full description and characterization of a plasmid from A. ruhlandii which was maintained over time. (Copyright © 2021 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu