| Autor: |
Ferreira GD; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil., Fernandes GMM; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil., Penteado C; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil., Cória VR; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil., Galbiatti-Dias ALDS; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil.; São José do Rio Preto Regional Medical School Foundation - FUNFARME, São José do Rio Preto, Brazil., Russo A; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil., Castanhole-Nunes MMU; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil.; São José do Rio Preto Regional Medical School Foundation - FUNFARME, São José do Rio Preto, Brazil., Silva RFD; Study Group of Liver Tumors - GETF, Surgery Department, São José do Rio Preto Medical School Fundation - FAMERP/FUNFARME, São José do Rio Preto, Brazil.; São José do Rio Preto Regional Medical School Foundation - FUNFARME, São José do Rio Preto, Brazil., Silva RCMAD; Study Group of Liver Tumors - GETF, Surgery Department, São José do Rio Preto Medical School Fundation - FAMERP/FUNFARME, São José do Rio Preto, Brazil.; São José do Rio Preto Regional Medical School Foundation - FUNFARME, São José do Rio Preto, Brazil., Pavarino ÉC; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil.; São José do Rio Preto Regional Medical School Foundation - FUNFARME, São José do Rio Preto, Brazil., Torreglosa Ruiz Cintra M; Department of Biological Sciences - DCB, Federal University of the Triângulo Mineiro - UFTM, Uberaba, Brazil., Goloni-Bertollo EM; Molecular Biology Department, Genetics and Molecular Biology Research Unit - UPGEM, São José do Rio Preto Medical School - FAMERP, São Jose do Rio Preto, Brazil.; São José do Rio Preto Regional Medical School Foundation - FUNFARME, São José do Rio Preto, Brazil. |
| Abstrakt: |
This study was performed to investigate the relationship between polymorphisms in microsomal epoxide hydrolase ( mEH ; Tyr113His and His139Arg substitution) and glutathione S-transferase ( GST ; GSTM1 deletion, GSTT1 deletion, and GSTP1 .Ala114Val substitution) and their correlation with clinico-histopathological features in hepatocellular carcinoma (HCC).We evaluated environmental risk factors and genetic alterations in 556 individuals (86 cases and 470 controls). PCR multiplex for GSTM1 and GSTT1 , polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for GSTP1 , and real-time PCR for mEH were performed. Statistical analyses were performed using multiple logistic regression tests.Age over 48 years ( p < 0.001) and alcohol consumption ( p = 0.021) were the predictors of increased risk of developing HCC. GSTP1 .Ala114Val for all regression models ( p < 0.05), except the recessive model, and the GSTT1 null genotype (odds ratio [OR] = 0.43, 95% confidence interval [CI] = 0.21-0.87, p = 0.019) were predictors of an increased risk of developing HCC. Polymorphic GSTT1, GSTM1 , GSTP1. Ala114Val, and mEH .His139Arg and wild-type mEH.Tyr113His (OR = 5.04; 95% CI = 1.59-16.04; p = 0.006) were associated with HCC.Age over 48 years, alcohol consumption, and the presence of polymorphic variants of GSTP1 and GSTT1 were associated with the risk of developing HCC. |
