Immunoprotective inference of experimental chronic Trichinella spiralis infection on house dust mites induced allergic airway remodeling.

Autor: Elmehy DA; Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt. Electronic address: dalia.elmehy@med.tanta.edu.eg., Abdelhai DI; Clinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Elkholy RA; Clinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Elkelany MM; Histology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Tahoon DM; Pharmacology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Elkholy RA; Pharmacology Department, Faculty of Medicine, Tanta University, Tanta, Egypt; Pharmacology Department, School of Medicine, Badr University, Cairo, Egypt., Soliman NA; Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Saad MAH; Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., El-Ebiary AA; Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Tanta University, Tanta, Egypt., Gamea GA; Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Aug; Vol. 220, pp. 105934. Date of Electronic Publication: 2021 Apr 23.
DOI: 10.1016/j.actatropica.2021.105934
Abstrakt: Allergic bronchial asthma is characterized by chronic inflammation of the respiratory airways mediated by T-helper 2 (Th2), Th17 and their cytokines. Although most asthmatic patients suffer from allergic airway remodeling (AAR), aggressive anti-allergic treatment failed to reverse it. The hygiene hypothesis illuminated the counter relationship between allergy and helminthic infections. The immune system is modulated by Trichinella spiralis (T. spiralis) infection to maintain homeostasis. Therefore, this work aimed to investigate the impact of chronic T. spiralis infection on induced AAR in C57BL/6 mice sensitized by house dust mites (HDM) allergens. Forty mice were divided into 3 groups: I (10 healthy mice), IΙ (15 HDM sensitized mice), and ΙΙI (15 T. spiralis chronically infected mice and sensitized with HDM allergens). The assessment aimed to evaluate the effects of regulatory CD4+CD25+FOXP3+ cells (Tregs) and their cytokines comparative to hypersensitivity mediated cytokines. Chronic T. spiralis infection effectively prevented the host's AAR. This result was evidenced by upregulated Tregs in blood by flow cytometric analysis and increased interleukin-10 (IL-10) levels in bronchoalveolar lavage (BAL) by Enzyme linked immunosorbent assay (ELISA) as well as improved lung histopathological changes. Also, serum HDM specific immunoglobulin E (IgE), BAL eosinophils, BAL IL-5 levels, and IL-17 gene expression in lung tissues were significantly reduced in T. spiralis chronically infected mice. In conclusion, the immune response in chronic T. spiralis infection could provide a promising mechanistic tool for protection against AAR, which paves the way for innovative preventive measures of other immunological disorders.
(Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: