Changes in genotoxicity, inflammatory and oxidative stress parameters of workers in marble processing plants.

Autor: Beci̇t M; Department of Pharmacology, Faculty of Pharmacy, Ataturk University, Erzurum, 25240, Turkey., Çi̇lekar Ş; Department of Chest Diseases, Faculty of Medicine, Afyonkarahisar University of Health Sciences, Afyonkarahisar, 03030, Turkey., Başaran MM; Department of Otorhinolaryngology, Faculty of Medicine, Kafkas University, Kars, 36100, Turkey., Koca HB; Department of Medical Biochemistry, Faculty of Medicine, Afyonkarahisar University of Health Sciences, Afyonkarahisar, 03030, Turkey., Çeli̇k S; Department of Medical Biochemistry, Faculty of Medicine, Afyonkarahisar University of Health Sciences, Afyonkarahisar, 03030, Turkey., Di̇lsi̇z SA; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, Ankara, 06100, Turkey. Electronic address: sevtapay@hacettepe.edu.tr.
Jazyk: angličtina
Zdroj: Environmental research [Environ Res] 2021 Jun; Vol. 197, pp. 111209. Date of Electronic Publication: 2021 Apr 23.
DOI: 10.1016/j.envres.2021.111209
Abstrakt: Workers in marble processing plants are at high risk of exposure to high levels of marble dust containing silica, but there are limited studies evaluating the genotoxicity and oxidative stress parameters of workers occupationally exposed to marble dust. In this study, we aimed to clarify how marble dust affects genotoxicity and immunotoxicity mechanisms alongside oxidative stress in the workers in the marble processing plants of Iscehisar, Turkey. The oxidative stress and immune system parameters were determined spectrophotometrically using commercial kits. Genotoxicity was evaluated by Comet and micronucleus (MN) assays in the lymphocytes and buccal cells, respectively. The enzyme activities of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase, and the levels of glutathione, and Clara cell secretory protein CC16 in workers (n = 48) were significantly lower than in controls (n = 41), whereas the levels of malondialdehyde, 8-oxo-7,8-dihydro-2'-deoxyguanosine, tumor necrosis factor-alpha, interleukin-1beta were significantly higher in workers. DNA damage in workers were significantly higher than in controls and there was a clear correlation between the increase in DNA damage and the duration of exposure. Marble workers had significantly higher MN frequencies when compared to controls. The results indicate the possibility of immunotoxic and genotoxic risks to workers in marble industry.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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