Dark adaptation and ability of pulse-amplitude modulated (PAM) fluorometry to identify nutrient limitation in the bloom-forming cyanobacterium, Microcystis aeruginosa (Kützing).

Autor: Perri KA; The State University of New York - College of Environmental Science and Forestry, Department of Chemistry, 1 Forestry Drive, 121 Jahn Lab, Syracuse, NY 13210, USA. Electronic address: kaperri@tamu.edu., Manning SR; University of Texas at Austin, Department of Molecular Biosciences, 205 W. 24th Street, Austin, TX 78712, USA., Watson SB; Trent University, School of Graduate Studies, 1600 West Bank Drive, Peterborough, ON K9L 0G2, Canada., Fowler NL; University of Texas at Austin, Department of Integrative Biology, 205 W. 24th Street, Austin, TX 78712, USA., Boyer GL; The State University of New York - College of Environmental Science and Forestry, Department of Chemistry, 1 Forestry Drive, 121 Jahn Lab, Syracuse, NY 13210, USA.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2021 Jun; Vol. 219, pp. 112186. Date of Electronic Publication: 2021 Apr 07.
DOI: 10.1016/j.jphotobiol.2021.112186
Abstrakt: Harmful algal blooms in inland waters are widely linked to excess phosphorus (P) loading, but increasing evidence shows that their growth and formation can also be influenced by nitrogen (N) and iron (Fe). Deficiency in N, P, and Fe differentially affects cellular photosystems and is manifested as changes in photosynthetic yield (F v /F m ). While F v /F m has been increasingly used as a rapid and convenient in situ gauge of nutrient deficiency, there are few rigorous comparisons of instrument sensitivity and ability to resolve specific nutrient stresses. This study evaluated the application of F v /F m to cyanobacteria using controlled experiments on a single isolate and tested three hypotheses: i) single F v /F m measurements taken with different PAM fluorometers can distinguish among limitation by different nutrients, ii) measurements of F v /F m made by the addition of DCMU are comparable to PAM fluorometers, and iii) dark adaptation is not necessary for reliable F v /F m measurements. We compared F v /F m taken from the bloom-forming Microcystis aeruginosa (UTEX LB 3037) grown in nutrient-replete treatment (R) and N-, P-, and Fe-limited treatments (LN, LP, LFe, respectively), using three pulse-amplitude modulated (PAM) fluorometers and the chemical photosynthesis inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and evaluated the effects of dark adaptation prior to PAM measurement. There were significant differences in F v /F m estimates among PAM fluorometers for light- versus dark-adapted cell suspensions over the whole experiment (21 days), which were all significantly higher than the DCMU-based measurements. However, dark adaptation had no effect on F v /F m when comparing PAM-based values across a single nutrient treatment. All F v /F m methods could distinguish LN and LP from R and LFe treatments but none were able to resolve LFe from R, or LN from LP cultures. These results indicated that for most PAM applications, dark adaptation is not necessary, and furthermore that single measurements of F v /F m do not provide a robust measurement of nutrient limitation in Microcystis aeruginosa UTEX LB 3037, and potentially other, common freshwater cyanobacteria.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: