Luffa operculata at a late period of gestation dysregulates melatonin and cytokines interfering with weight of dams and their male offspring.

Autor: Alves CDS; Programa de Pós-Graduação Em Patologia Ambiental e Experimental, Universidade Paulista - UNIP, Brazil., Frias HV; Programa de Pós-Graduação Em Patologia Ambiental e Experimental, Universidade Paulista - UNIP, Brazil., Bonamin LV; Programa de Pós-Graduação Em Patologia Ambiental e Experimental, Universidade Paulista - UNIP, Brazil., Correia MSF; Programa de Pós-Graduação Em Patologia Ambiental e Experimental, Universidade Paulista - UNIP, Brazil., Corrêa MG; Programa de Pós-Graduação Em Odontologia, Universidade Paulista - UNIP, Brazil., Bondan EF; Programa de Pós-Graduação Em Patologia Ambiental e Experimental, Universidade Paulista - UNIP, Brazil., de Fátima M Martins M; Departamento de Medicina Veterinária, Universidade Cruzeiro Do Sul, Brazil., Coelho CP; Universidade Santo Amaro - UNISA, Brazil., Bernardi MM; Programa de Pós-Graduação Em Patologia Ambiental e Experimental, Universidade Paulista - UNIP, Brazil; Programa de Pós-Graduação Em Odontologia, Universidade Paulista - UNIP, Brazil., Suffredini IB; Programa de Pós-Graduação Em Patologia Ambiental e Experimental, Universidade Paulista - UNIP, Brazil; Programa de Pós-Graduação Em Odontologia, Universidade Paulista - UNIP, Brazil; Núcleo de Pesquisas Em Biodiversidade, Laboratório de Extração, Universidade Paulista - UNIP, Brazil. Electronic address: ivana.suffredini@docente.unip.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2021 Jul 15; Vol. 275, pp. 113867. Date of Electronic Publication: 2021 Apr 21.
DOI: 10.1016/j.jep.2021.113867
Abstrakt: Ethnopharmacological Relevance: The tea made with the fruits of Luffa operculata (L.) Cogn. (Cucurbitaceae; EBN) is popularly used as abortive.
Aim of the Study: The present work aimed at accessing how the exposition of female Wistar rats to 1.0 mg/kg of EBN (experimental group, EG), or distilled water (control group, CG), by gavage, at gestational days (GD) 17-21 interfered with the reproductive performance, and with dams' behavior after weaning.
Materials and Methods: At post-natal day 2 (PND2), the number of male and female pups was evaluated, as well as their weight. After weaning (PND21), dams were euthanized, and their liver and kidneys were removed for histological and biochemical analyses, while the blood was used in the evaluation of cytokines IL-1α, IL-1β, IL-6 and TNF-α, corticosterone, adrenocorticotrophic hormone, melatonin, AST, ALT and creatinine levels.
Results and Discussion: Dams that were treated with EBN showed an anxiety-like behavior, weight loss at the end of gestation and weight gain at weaning, accompanied with a significant decrease in pro-inflammatory cytokines and in the melatonin level. No significant histological or biochemical alterations have occurred in the liver or kidneys. The number of female pups was significantly higher in the EG. The male pups showed weight gain at PND60.
Conclusion: The presence of cucurbitacins is probably involved in the dysregulations that were found, due to their polycyclic steroid triterpene structure.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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