Oxygen releasing hydrogels for beta cell assisted therapy.

Autor: Toftdal MS; Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; Department of Stem Cell Delivery & Pharmacology, Novo Nordisk A/S, DK-2760 Måløv, Denmark., Taebnia N; Center for Intestinal Absorption and Transport of Biopharmaceuticals, Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark., Kadumudi FB; Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. Electronic address: fbka@dtu.dk., Andresen TL; Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; Center for Intestinal Absorption and Transport of Biopharmaceuticals, Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark., Frogne T; Department of Stem Cell Discovery, Novo Nordisk A/S, DK-2760 Måløv, Denmark., Winkel L; Department of Stem Cell Delivery & Pharmacology, Novo Nordisk A/S, DK-2760 Måløv, Denmark., Grunnet LG; Department of Stem Cell Delivery & Pharmacology, Novo Nordisk A/S, DK-2760 Måløv, Denmark., Dolatshahi-Pirouz A; Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; Center for Intestinal Absorption and Transport of Biopharmaceuticals, Department of Health Technology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark; Radboud Institute for Molecular Life Sciences, Department of Dentistry - Regenerative Biomaterials, Radboud University Medical Center, Philips van Leydenlaan 25, 6525EX Nijmegen, the Netherlands. Electronic address: aldo@dtu.dk.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2021 Jun 01; Vol. 602, pp. 120595. Date of Electronic Publication: 2021 Apr 20.
DOI: 10.1016/j.ijpharm.2021.120595
Abstrakt: Diabetes is a serious chronic disease, which globally affects more than 400 million patients. Beta cell therapy has potential to serve as an effective cure to type 1 diabetes and several studies have already shown promising results in this regard. One of the major obstacles in cell therapy, however, is the hypoxic environment that therapeutic cells are subjected to immediately after the transplantation. In this study, a new approach is presented, based on hydrogels composed of thiolated hyaluronic acid (tHA), 8-arm-Poly(ethylene glycol)-Acrylate (PEGA), and calcium peroxide (CPO) as an oxygen releasing system. Hydrogels containing 0, 7.5, and 30% CPO were prepared, and the presence of CPO was confirmed via FTIR and Alizarin Red within the network. Oxygen release kinetics were monitored over time, and the results revealed that the hydrogels containing 30% CPO could release oxygen for at least 30 h. All three combinations were found to be injectable and suitable for beta cell therapy based on their mechanical and rheological properties. Additionally, to investigate the functionality of the system, insulin secreting INS-1E reporter cell clusters were encapsulated, and their viability was evaluated, which showed that CPO incorporation enhanced cell survival for at least three days.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE