Diagnostic potential of hypoxia-induced genes in liquid biopsies of breast cancer patients.

Autor:	Peiró CHF; Laboratório de Análises Clínicas do Centro Universitário Saúde ABC, Faculdade e Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil., Perez MM; Laboratório de Análises Clínicas do Centro Universitário Saúde ABC, Faculdade e Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil., de Aquino GSA; Laboratório de Análises Clínicas do Centro Universitário Saúde ABC, Faculdade e Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil., Encinas JFA; Laboratório de Análises Clínicas do Centro Universitário Saúde ABC, Faculdade e Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil., Sousa LVA; Laboratório de Epidemiologia e Análise de Dados, FMABC, Santo André, SP, Brazil., da Veiga GL; Laboratório de Análises Clínicas do Centro Universitário Saúde ABC, Faculdade e Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil., Del Giglio A; Departamento de Oncologia e Hematologia do Centro Universitário Saúde ABC-Faculdade de Medicina do ABC, Santo André, SP, Brazil., Fonseca FLA; Laboratório de Análises Clínicas do Centro Universitário Saúde ABC, Faculdade e Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil.; Instituto de Ciências Farmacêuticas, Universidade Federal de São Paulo (UNIFESP), Diadema, SP, Brazil., da Costa Aguiar Alves B; Laboratório de Análises Clínicas do Centro Universitário Saúde ABC, Faculdade e Medicina do ABC, Av. Lauro Gomes, 2000, Santo André, SP, CEP 09060-870, Brazil. bcaalves@uol.com.br.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2021 Apr 22; Vol. 11 (1), pp. 8724. Date of Electronic Publication: 2021 Apr 22.
DOI:	10.1038/s41598-021-87897-2
Abstrakt:	In tumor cells, higher expression of glucose transporter proteins (GLUT) and carbonic anhydrases (CAIX) genes is influenced by hypoxia-induced factors (HIF).Thus, we aimed to study the expression profile of these markers in sequential peripheral blood collections performed in breast cancer patients in order to verify their predictive potential in liquid biopsies. Gene expressions were analyzed by qPCR in tumor and blood samples from 125 patients and 25 healthy women. Differential expression was determined by the 2(-ΔCq) method. Expression of HIF-1α and GLUT1 in the blood of breast cancer patients is significantly higher (90-91 and 160-161 fold increased expression, respectively; p < 0.0001) than that found in healthy women. Their diagnostic power was confirmed by ROC curve. CAIX is also more expressed in breast cancer women blood, but its expression was detected only in a few samples. But none of these genes could be considered predictive markers. Therefore, evaluation of the expression of HIF-1α and GLUT1 in blood may be a useful laboratory tool to complement the diagnosis of breast cancer, in addition to being useful for follow-up of patients and of women with a family history of breast cancer.
Databáze:	MEDLINE
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