Inducible nitric oxide synthase (iNOS) mediates ethanol-induced redox imbalance and upregulation of inflammatory cytokines in the kidney.

Autor: da Silva CBP; Laboratório de Farmacologia, DEPCH, Escola de Enfermagem de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil.; Programa de Pós-Graduação em Toxicologia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil., Ceron CS; Departamento de Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil., Mendes AS; Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil., de Martinis BS; Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil., Castro MM; Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil., Tirapelli CR; Laboratório de Farmacologia, DEPCH, Escola de Enfermagem de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil.
Jazyk: angličtina
Zdroj: Canadian journal of physiology and pharmacology [Can J Physiol Pharmacol] 2021 Oct; Vol. 99 (10), pp. 1016-1025. Date of Electronic Publication: 2021 Apr 22.
DOI: 10.1139/cjpp-2021-0108
Abstrakt: Overexpression of the inducible isoform of the enzyme nitric oxide synthase (iNOS) has been associated to pathological processes in the kidney. Ethanol consumption induces the renal expression of iNOS; however, the contribution of this enzyme to the deleterious effects of ethanol in the kidney remains elusive. We examined whether iNOS plays a role in the renal dysfunction and oxidative stress induced by ethanol consumption. With this purpose, male C57BL/6 wild-type (WT) or iNOS-deficient (iNOS -/- ) mice were treated with ethanol (20% v / v ) for 10 weeks. Treatment with ethanol increased the expression of Nox4 as well as the concentration of thiobarbituric acid reactive substances and the levels of tumor necrosis factor α in the renal cortex of WT but not iNOS -/- mice. Augmented serum levels of creatinine and increased systolic blood pressure were found in WT and iNOS -/- mice treated with ethanol. WT mice treated with ethanol showed increased production of reactive oxygen species and myeloperoxidase activity, but these responses were attenuated in iNOS -/- mice. We concluded that iNOS played a role in ethanol-induced oxidative stress and pro-inflammatory cytokine production in the kidney. These are mechanisms that may contribute to the renal toxicity induced by ethanol.
Databáze: MEDLINE
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