Cytotoxicity and DNA damage evaluation of TiO 2 and ZnO nanoparticles. Uptake in lung cells in culture.

Autor: Freire K; CEQUINOR, (CONICET-UNLP), Bv. 120 N 1465, La Plata, Argentina., Ordóñez Ramos F; Escuela de Química, Facultad de Ciencias, Universidad Nacional de Colombia, sede Medellín. Cra 65 #59A -110, Medellín, Colombia., Soria DB; CEQUINOR, (CONICET-UNLP), Bv. 120 N 1465, La Plata, Argentina., Pabón Gelves E; Escuela de Química, Facultad de Ciencias, Universidad Nacional de Colombia, sede Medellín. Cra 65 #59A -110, Medellín, Colombia., Di Virgilio AL; CEQUINOR, (CONICET-UNLP), Bv. 120 N 1465, La Plata, Argentina.
Jazyk: angličtina
Zdroj: Toxicology research [Toxicol Res (Camb)] 2021 Mar 09; Vol. 10 (2), pp. 192-202. Date of Electronic Publication: 2021 Mar 09 (Print Publication: 2021).
DOI: 10.1093/toxres/tfaa112
Abstrakt: The cytotoxicity and DNA damage of titanium dioxide and zinc oxide nanoparticles (TiO 2 and ZnO NPs) have been studied in a human lung carcinoma cell line (A549) after 24 h exposure. TiO 2 and ZnO NPs had mean diameters of 12.9 ± 2.8 and 24.1 ± 8.0 nm, respectively. ZnO NPs reduced cell viability from 250 μg/mL, increasing reactive oxygen species (ROS) and decreased GSH/GSSG ratio. The comet assay detected DNA damage from 50 μg/mL. TiO 2 NPs induced cytotoxicity and DNA damage from 50 to 100 μg/mL, respectively, along with a decrease of the GSH/GSSG ratio. Both particles were found inside the cells, within membrane-bound vesicles. The internalization mechanism is promoted partially by caveolae-mediated endocytosis and, in the case of TiO 2 NPs, also by macropinocytosis.
(© The Author(s) 2021. Published by Oxford University Press.)
Databáze: MEDLINE
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