Single-Dilution COVID-19 Antibody Test with Qualitative and Quantitative Readouts.

Autor:	Bortz RH 3rd; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA., Florez C; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, New York, USA., Laudermilch E; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA., Wirchnianski AS; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Lasso G; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA., Malonis RJ; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Georgiev GI; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Vergnolle O; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Herrera NG; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Morano NC; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Campbell ST; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Orner EP; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Mengotto A; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Dieterle ME; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA., Fels JM; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA., Haslwanter D; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA., Jangra RK; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA., Celikgil A; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Kimmel D; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Lee JH; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Mariano MC; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Nakouzi A; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Quiroz J; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Rivera J; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Szymczak WA; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Tong K; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Barnhill J; Department of Chemistry and Life Science, United States Military Academy at West Point, West Point, New York, USA., Forsell MNE; Department of Clinical Microbiology, Umeå University, Umeå, Sweden., Ahlm C; Department of Clinical Microbiology, Umeå University, Umeå, Sweden., Stein DT; Montefiore Medical Center, Bronx, New York, USA.; Division of Endocrinology and Diabetes, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA., Pirofski LA; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Goldstein DY; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Garforth SJ; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Almo SC; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA., Daily JP; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Prystowsky MB; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Faix JD; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Fox AS; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA.; Montefiore Medical Center, Bronx, New York, USA., Weiss LM; Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA louis.weiss@einsteinmed.org jon.lai@einsteinmed.org kartik.chandran@einsteinmed.org.; Montefiore Medical Center, Bronx, New York, USA., Lai JR; Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York, USA louis.weiss@einsteinmed.org jon.lai@einsteinmed.org kartik.chandran@einsteinmed.org., Chandran K; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA louis.weiss@einsteinmed.org jon.lai@einsteinmed.org kartik.chandran@einsteinmed.org.
Jazyk:	angličtina
Zdroj:	MSphere [mSphere] 2021 Apr 21; Vol. 6 (2). Date of Electronic Publication: 2021 Apr 21.
DOI:	10.1128/mSphere.00224-21
Abstrakt:	The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior SARS-CoV-2 infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity. We further show that this sensitive test affords the estimation of viral spike-specific IgG titers from a single sample measurement, thereby providing a simple and scalable method to measure the strength of an individual's immune response. The accuracy, adaptability, and cost-effectiveness of this test make it an excellent option for clinical deployment in the ongoing COVID-19 pandemic. IMPORTANCE Serological surveillance has become an important public health tool during the COVID-19 pandemic. Detection of protective antibodies and seroconversion after SARS-CoV-2 infection or vaccination can help guide patient care plans and public health policies. Serology tests can detect antibodies against past infections; consequently, they can help overcome the shortcomings of molecular tests, which can detect only active infections. This is important, especially when considering that many COVID-19 patients are asymptomatic. In this study, we describe an enzyme-linked immunosorbent assay (ELISA)-based qualitative and quantitative serology test developed to measure IgG and IgA antibodies against the SARS-CoV-2 spike glycoprotein. The test can be deployed using commonly available laboratory reagents and equipment and displays high specificity and sensitivity. Furthermore, we demonstrate that IgG titers in patient samples can be estimated from a single measurement, enabling the assay's use in high-throughput clinical environments. (Copyright © 2021 Bortz et al.)
Databáze:	MEDLINE
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