The effect of intratesticular dexpanthenol on experimentally-induced testicular ischaemia/reperfusion injury.

Autor: Aydın A; NEÜ Meram Medicine Faculty Department of Urology, Konya, Turkey. Electronic address: aydinarif@gmail.com., Sönmez MG; NEÜ Meram Medicine Faculty Department of Urology, Konya, Turkey. Electronic address: drgiraysonmez@gmail.com., Ecer G; NEÜ Meram Medicine Faculty Department of Urology, Konya, Turkey. Electronic address: ecergokhan@gmail.com., Kılınç F; NEÜ Meram Medicine Faculty Department of Pathology, Konya, Turkey. Electronic address: drfahriyek@gmail.com., Kocabaş R; NEÜ Meram Medicine Faculty Konüdam Exp. Medicine&App. Res. Center, Konya, Turkey. Electronic address: drrahim42@gmail.com., Atılgan AE; Faculty of Medicine, Department of Urogynecology, Medipol Mega University Hospital, Bağcılar, İstanbul, Turkey. Electronic address: dradeviye@gmail.com., Oltulu P; NEÜ Meram Medicine Faculty Department of Pathology, Konya, Turkey. Electronic address: drpembe@yahoo.com., Balasar M; NEÜ Meram Medicine Faculty Department of Urology, Konya, Turkey. Electronic address: drbalasar@gmail.com.
Jazyk: angličtina
Zdroj: Journal of pediatric urology [J Pediatr Urol] 2021 Aug; Vol. 17 (4), pp. 440.e1-440.e7. Date of Electronic Publication: 2021 Apr 08.
DOI: 10.1016/j.jpurol.2021.03.031
Abstrakt: Introduction: Testis torsion is a urological emergency and a serious situation that may cause testis atrophy, testicular dysfunction and infertility due to ischaemia/reperfusion(I/R) injury even with early intervention.
Objective: To assess the protective effect of dexpanthenol administered intratesticular after detorsion against testis I/R injury.
Study Design: Twenty-seven rats were randomly divided into 3 groups containing 9 rats each. The 1st group comprised the sham group with no procedure performed. The 2nd group had only torsion applied, while the 3rd group had torsion + dexpanthenol applied. Rats had 720° clockwise rotation applied to the left testis. After 2 h of ischaemia, testes were de-torsioned and the dexpanthenol group had 500 mg/kg dexpanthenol administered intratesticular after detorsion. After 4 h of reperfusion, rats had blood samples taken and orchiectomy was performed for histologic assessment.
Results: A significant difference was detected in all parameters [necrosis(p:<0.001), Cosentino grade (p < 0.001), congestion (p:0.005), fibrosis (p:<0.001), interstitial oedema (p:0.017), JTBS score (p:<0.001), apoptosis (p < 0.001) and testosterone levels (p:0.006)] when the sham, torsion, and torsion + dexpanthenol groups were compared. Significant differences were observed for fibrosis (p:0.010), Cosentino score (p < 0.001), JTBS score (p:<0.001), apoptosis (p:0.001) and total testosterone levels (p:0.013) when torsion and torsion + dexpanthenol groups were compared. The torsion + dexpanthenol group was identified to have more preservation of testis function observed histologically and hormonally compared to the torsion group.
Discussion: Dexpanthenol is used in many areas due to both epithelizing and antioxidant effects and lack of clear side effects. In spite of use of many chemical and biological agents to protect against testis I/R injury, none have entered routine use. This study showed that dexpanthenol, which can be easily injected intratesticular during detorsion surgery, has protective effect against histological and functional injury that may develop linked to testis I/R injury. The main limitations of the study are short duration of follow-up due to being a rat experiment and lack of comparison of lipid peroxidation products.
Conclusion: This study identified that dexpanthenol with clinically easy use by intratesticular injection after detorsion during surgery had a protective effect against histological and functional injury that will develop linked to I/R injury in the testis.
Competing Interests: Conflict of interest None of the authors have any potential conflict of interest.
(Copyright © 2021 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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