Peptidomics of an industrial gluten-free barley malt beer and its non-gluten-free counterpart: Characterisation and immunogenicity.

Autor: Watson HG; Ghent University, Faculty of Bioscience Engineering, Department of Biotechnology, Valentin Vaerwyckweg 1, Ghent 9000, Belgium. Electronic address: Hellen.Watson@UGent.be., Decloedt AI; Ghent University, Faculty of Veterinary Medicine, Laboratory of Chemical Analysis, Salisburylaan 133, Merelbeke 9820, Belgium., Hemeryck LY; Ghent University, Faculty of Veterinary Medicine, Laboratory of Chemical Analysis, Salisburylaan 133, Merelbeke 9820, Belgium., Van Landschoot A; Ghent University, Faculty of Bioscience Engineering, Department of Biotechnology, Valentin Vaerwyckweg 1, Ghent 9000, Belgium., Prenni J; Colorado State University, Proteomics & Metabolomics Facility, 2021 Campus Delivery, Fort Collins, CO 80523, USA.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2021 Sep 01; Vol. 355, pp. 129597. Date of Electronic Publication: 2021 Mar 18.
DOI: 10.1016/j.foodchem.2021.129597
Abstrakt: Recent research suggests that gluten-free beers by prolyl-endopeptidase treatment may not be safe for coeliac disease (CD) patients. Therefore, the gluten peptidome of an industrial gluten-free prolyl-endopeptidase treated malt beer (<10 ppm gluten) was compared to its untreated counterpart (58 ppm gluten) as a reference. NanoLC-HRMS analysis revealed the presence of 155 and 158 gluten peptides in the treated and reference beer, respectively. Characterisation of the peptides in treated beer showed that prolyl-endopeptidase activity was not complete with many peptides containing (multiple) internal proline-residues. Yet, prolyl-endopeptidase treatment did eliminate complete CD-immunogenic motifs, however, 18 peptides still contained partial, and potentially unsafe, motifs. In the reference beer respectively 7 and 37 gluten peptides carried (multiple) complete and/or partial CD-immunogenic motifs. Worrying is that many of these partial immunogenic gluten peptides do not contain a recognition epitope for the R5-antibody and would be overlooked in the current ELISA analysis for gluten quantification.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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