Distinguishing Rectal Cancer from Colon Cancer Based on the Support Vector Machine Method and RNA-sequencing Data.

Autor: Zhang Y; Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China., Wu Y; Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China., Gong ZY; Shanghai Yunying Medical Technology Co., Ltd., Shanghai, 201612, China.; Jiaxing Yunying Medical Inspection Co., Ltd., Jiaxing, 314000, China., Ye HD; Shanghai Yunying Medical Technology Co., Ltd., Shanghai, 201612, China.; Jiaxing Yunying Medical Inspection Co., Ltd., Jiaxing, 314000, China., Zhao XK; Shanghai Yunying Medical Technology Co., Ltd., Shanghai, 201612, China.; Jiaxing Yunying Medical Inspection Co., Ltd., Jiaxing, 314000, China., Li JY; Shanghai Yunying Medical Technology Co., Ltd., Shanghai, 201612, China.; Jiaxing Yunying Medical Inspection Co., Ltd., Jiaxing, 314000, China., Zhang XM; Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China., Li S; Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China., Zhu W; School of Medicine, Jiangsu University, Zhenjiang, 212013, China., Wang M; School of Medicine, Jiangsu University, Zhenjiang, 212013, China., Liang GY; School of Public Health, Southeast University, Nanjing, 211189, China., Liu Y; Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China., Guan X; Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China., Zhang DY; Shanghai Yunying Medical Technology Co., Ltd., Shanghai, 201612, China.; Jiaxing Yunying Medical Inspection Co., Ltd., Jiaxing, 314000, China., Shen B; Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, China. shenbo987@126.com.
Jazyk: angličtina
Zdroj: Current medical science [Curr Med Sci] 2021 Apr; Vol. 41 (2), pp. 368-374. Date of Electronic Publication: 2021 Apr 20.
DOI: 10.1007/s11596-021-2356-8
Abstrakt: Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide. Several studies have indicated that rectal cancer is significantly different from colon cancer in terms of treatment, prognosis, and metastasis. Recently, the differential mRNA expression of colon cancer and rectal cancer has received a great deal of attention. The current study aimed to identify significant differences between colon cancer and rectal cancer based on RNA sequencing (RNA-seq) data via support vector machines (SVM). Here, 393 CRC samples from the The Cancer Genome Atlas (TCGA) database were investigated, including 298 patients with colon cancer and 95 with rectal cancer. Following the random forest (RF) analysis of the mRNA expression data, 96 genes such as HOXB13, PRAC, and BCLAF1 were identified and utilized to build the SVM classification model with the Leave-One-Out Cross-validation (LOOCV) algorithm. In the training (n=196) and the validation cohorts (n=197), the accuracy (82.1 % and 82.2 %, respectively) and the AUC (0.87 and 0.91, respectively) indicated that the established optimal SVM classification model distinguished colon cancer from rectal cancer reasonably. However, additional experiments are required to validate the predicted gene expression levels and functions.
Databáze: MEDLINE
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