Identification of a micropeptide and multiple secondary cell genes that modulate Drosophila male reproductive success.

Autor:	Immarigeon C; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; clem.immarigeon@gmail.com robert.maeda@unige.ch., Frei Y; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland., Delbare SYN; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703., Gligorov D; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland., Machado Almeida P; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland., Grey J; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703., Fabbro L; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland., Nagoshi E; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland., Billeter JC; Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen 9700 CC, The Netherlands., Wolfner MF; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853-2703., Karch F; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland., Maeda RK; Department of Genetics and Evolution, Sciences III, University of Geneva, 1211 Geneva 4, Switzerland; clem.immarigeon@gmail.com robert.maeda@unige.ch.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Apr 13; Vol. 118 (15).
DOI:	10.1073/pnas.2001897118
Abstrakt:	Even in well-characterized genomes, many transcripts are considered noncoding RNAs (ncRNAs) simply due to the absence of large open reading frames (ORFs). However, it is now becoming clear that many small ORFs (smORFs) produce peptides with important biological functions. In the process of characterizing the ribosome-bound transcriptome of an important cell type of the seminal fluid-producing accessory gland of Drosophila melanogaster , we detected an RNA, previously thought to be noncoding, called male-specific abdominal ( msa ). Notably, msa is nested in the HOX gene cluster of the Bithorax complex and is known to contain a micro-RNA within one of its introns. We find that this RNA encodes a "micropeptide" (9 or 20 amino acids, MSAmiP) that is expressed exclusively in the secondary cells of the male accessory gland, where it seems to accumulate in nuclei. Importantly, loss of function of this micropeptide causes defects in sperm competition. In addition to bringing insights into the biology of a rare cell type, this work underlines the importance of small peptides, a class of molecules that is now emerging as important actors in complex biological processes. Competing Interests: The authors declare no competing interest. (Copyright © 2021 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
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