Synergistic activity of diclofenac sodium with oxacillin against planktonic cells and biofilm of methicillin-resistant Staphylococcus aureus strains.

Autor: Queiroz HA; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., da Silva CR; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., de Andrade Neto JB; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.; University Center Christus, Fortaleza, CE, Brazil., do Av Sá LG; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.; University Center Christus, Fortaleza, CE, Brazil., do Nascimento FB; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., Moreno LS; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., Barroso FD; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., da Silva LJ; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., Cândido TM; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., de Oliveira LC; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., de Mesquita JR; St. Joseph Hospital for Infectious Diseases, Fortaleza, CE, Brazil., de Moraes MO; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., Cavalcanti BC; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil., Nobre Júnior HV; Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
Jazyk: angličtina
Zdroj: Future microbiology [Future Microbiol] 2021 Apr; Vol. 16, pp. 375-387. Date of Electronic Publication: 2021 Apr 19.
DOI: 10.2217/fmb-2020-0095
Abstrakt: Aim: To evaluate the activity of diclofenac sodium and synergism with oxacillin against clinical strains of SARM in plactonic cells, antibiofilm and biofilm. Materials & methods: Synergism activity was assessed using the fractional inhibitory concentration index and its possible mechanism of action by flow cytometry. Results: The synergistic activity of diclofenac sodium with oxacillin was observed against plactonic cells, antibiofilm and in biofilm formed from clinical methicillin-resistant Staphylococcus aureus strains. Conclusion: This combination caused damage to the integrity of the membrane and ruptures in the DNA of the cells, leading to apoptosis.
Databáze: MEDLINE