| Autor: |
Cornacini MR; Departamento de Ciências Químicas e Biológicas, Instituto de Biociências, UNESP - Universidade Estadual Paulista, Botucatu, Brazil., Manoel RO; Instituto de Biotecnologia (IBTEC), UNESP - Universidade Estadual Paulista, Botucatu, Brazil. rickom.isa@gmail.com., Alcantara MAM; Departamento de Ciências Químicas e Biológicas, Instituto de Biociências, UNESP - Universidade Estadual Paulista, Botucatu, Brazil., Moraes MLT; Departamento de Fitotecnia, Tecnologia de Alimentos e Sócio Economia, Faculdade de Engenharia de Ilha Solteira, UNESP - Universidade Estadual Paulista, Ilha Solteira, Brazil., Silva EAA; Departamento de Produção Vegetal, Faculdade de Ciências Agronômicas, UNESP - Universidade Estadual Paulista, Botucatu, Brazil., Pereira Neto LG; Embrapa Recursos Genéticos e Biotecnologia (Cenargen), Brasilia, Brazil., Sebbenn AM; Instituto Florestal de São Paulo, Piracicaba, Brazil., Rossini BC; Departamento de Ciências Químicas e Biológicas, Instituto de Biociências, UNESP - Universidade Estadual Paulista, Botucatu, Brazil.; Instituto de Biotecnologia (IBTEC), UNESP - Universidade Estadual Paulista, Botucatu, Brazil., Marino CL; Departamento de Ciências Químicas e Biológicas, Instituto de Biociências, UNESP - Universidade Estadual Paulista, Botucatu, Brazil.; Instituto de Biotecnologia (IBTEC), UNESP - Universidade Estadual Paulista, Botucatu, Brazil. |
| Abstrakt: |
Astronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (G ST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. |
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