Prednisone is genotoxic in mice and Drosophila melanogaster.

Autor: de Oliveira LC; Laboratory of Radiobiology and Mutagenesis, Department of Genetics, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil., de Melo Bisneto AV; Laboratory of Radiobiology and Mutagenesis, Department of Genetics, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil., Puga SC; Laboratory of Radiobiology and Mutagenesis, Department of Genetics, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil., Fernandes AS; Laboratory of Radiobiology and Mutagenesis, Department of Genetics, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil., Véras JH; Laboratory of Radiobiology and Mutagenesis, Department of Genetics, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil., Cardoso CG; Laboratory of Radiobiology and Mutagenesis, Department of Histology, Embryology and Cell Biology, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil., Ribeiro E Silva C; Laboratory of Radiobiology and Mutagenesis, Department of Morphology, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil., Carneiro CC; Department of Biomedicine, School of Medical, Biomedical and Pharmaceutical Sciences, Pontifícia Universidade Católica de Goiás, 74605-010, Goiânia, GO, Brazil., Chen-Chen L; Laboratory of Radiobiology and Mutagenesis, Department of Genetics, Institute of Biological Sciences, Campus Samambaia, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil. Electronic address: chenleego@yahoo.com.br.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2021 May; Vol. 865, pp. 503334. Date of Electronic Publication: 2021 Feb 14.
DOI: 10.1016/j.mrgentox.2021.503334
Abstrakt: Prednisone (PD) is one of the most commonly used corticosteroids in immunosuppressive therapy for patients with autoimmune diseases and transplants. Chronic use of corticosteroids is associated with several side effects and an increase in neoplasia. Since genotoxic effects are associated with an increased risk of cancer development, this study evaluated the genotoxic and cytotoxic activities of PD using the SMART/wing assay in Drosophila melanogaster and the micronucleus test and comet assay in mouse bone marrow cells. Further, the toxic effects of PD on mouse organ tissues were assessed using histopathological analyses. In the SMART/wing assay, PD showed a significant genotoxic activity at all concentrations tested (0.375, 0.75, 1.5, and 2.0 mg/mL) compared to the negative control (p < 0.05). The micronucleus test and comet assay also showed an elevated genotoxicity of PD at all treatment conditions (24, 48, and 120 h with doses ranging from 0.5 to 1.5 mg/kg) compared to the negative control (p < 0.05). The histopathological analyses did not show toxicity of PD in mouse cells and tissues. Therefore, our results demonstrate that PD is a potent genotoxic immunosuppressant in mice and D. melanogaster cells. Somatic recombination was the primary contributor (46%-82%) to the induced genotoxicity observed in the SMART test.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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