Evaluation of the genotoxic and cytotoxic effects of exposure to the herbicide 2,4-dichlorophenoxyacetic acid in Astyanax lacustris (Pisces, Characidae) and the potential for its removal from contaminated water using a biosorbent.

Autor: Zafra-Lemos L; Department of Biotechnology, Genetics and Cellular Biology, Maringá State University, Av. Colombo, 5790 - Zona 7, Maringá, Paraná, Brazil. Electronic address: layonzafra@hotmail.com., Cusioli LF; Department of Chemical Engineering, Maringá State University, Av. Colombo, 5790 - Zona 7, Maringá, Paraná, Brazil., Bergamasco R; Department of Chemical Engineering, Maringá State University, Av. Colombo, 5790 - Zona 7, Maringá, Paraná, Brazil., Borin-Carvalho LA; Department of Biotechnology, Genetics and Cellular Biology, Maringá State University, Av. Colombo, 5790 - Zona 7, Maringá, Paraná, Brazil., Portela-Castro ALB; Department of Biotechnology, Genetics and Cellular Biology, Maringá State University, Av. Colombo, 5790 - Zona 7, Maringá, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2021 May; Vol. 865, pp. 503335. Date of Electronic Publication: 2021 Feb 13.
DOI: 10.1016/j.mrgentox.2021.503335
Abstrakt: The genotoxic and cytotoxic effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on specimens of Astyanax lacustris were evaluated using different biomarkers. Additionally, this study evaluated the efficiency of an activated carbon filter made from the husks green coconut, which was used as a biosorbent to remove 2,4-D dissolved in the water, and the potential effectiveness of this procedure for the reduction of the toxic effects of this compound on A. lacustris. Three sublethal concentrations of 2,4-D (10, 20, and 40 mg L -1 ) were tested over 24, 48, and 72 h, and their effects on Astyanax lacustris were evaluated using chromosomal aberration test, the mitotic index, the frequency of micronuclei and nuclear alterations, and the comet assay. Exposure to 2,4-D increased the frequency of chromosomal aberrations, reduced the mitotic index, and caused significant levels of nuclear modification in some of the treatments, in comparison with the negative control. The comet assay revealed DNA damage (classes 1-3) at all 2,4-D concentrations, reaching significant levels in the 20 mg L -1 (48 h) and 40 mg L -1 (72 h) treatments. The coconut husk biosorbent was highly effective for the removal of 2,4-D and the fish exposed to the water decontaminated by this filter had low levels of cellular alteration. The findings of the present study demonstrated, for the first time, the genotoxic and cytotoxic effects of 2,4-D in Astyanax lacustris, as well as suggests the potential application of a biosorbent for the effective decontamination of water contaminated with pesticides.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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