Immunophenotypic characterization of TCR γδ T cells and MAIT cells in HIV-infected individuals developing Hodgkin's lymphoma.

Autor: Muller CKS; Department of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland., Spagnuolo J; Department of Infectious Diseases and Hospital Hygiene, University Hospital of Basel, Basel, Switzerland., Audigé A; Institute of Medical Virology, University of Zurich, Zurich, Switzerland., Chancellor A; Department of Infectious Diseases and Hospital Hygiene, University Hospital of Basel, Basel, Switzerland., Russenberger D; Department of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland., Scherrer AU; Department of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland., Hoffmann M; Division of Infectious Diseases and Infection Control, Cantonal Hospital, St. Gallen, Switzerland., Kouyos R; Department of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland., Battegay M; Department of Infectious Diseases and Hospital Hygiene, University Hospital of Basel, Basel, Switzerland., De Libero G; Department of Infectious Diseases and Hospital Hygiene, University Hospital of Basel, Basel, Switzerland., Speck RF; Department of Infectious Diseases and Hospital Epidemiology, University Hospital of Zurich, University of Zurich, Zurich, Switzerland. roberto.speck@usz.ch.
Jazyk: angličtina
Zdroj: Infectious agents and cancer [Infect Agent Cancer] 2021 Apr 17; Vol. 16 (1), pp. 24. Date of Electronic Publication: 2021 Apr 17.
DOI: 10.1186/s13027-021-00365-4
Abstrakt: Background: Despite successful combined antiretroviral therapy (cART), the risk of non-AIDS defining cancers (NADCs) remains higher for HIV-infected individuals than the general population. The reason for this increase is highly disputed. Here, we hypothesized that T-cell receptor (TCR) γδ cells and/or mucosal-associated invariant T (MAIT) cells might be associated with the increased risk of NADCs. γδ T cells and MAIT cells both serve as a link between the adaptive and the innate immune system, and also to exert direct anti-viral and anti-tumor activity.
Methods: We performed a longitudinal phenotypic characterization of TCR γδ cells and MAIT cells in HIV-infected individuals developing Hodgkin's lymphoma (HL), the most common type of NADCs. Cryopreserved PBMCs of HIV-infected individuals developing HL, matched HIV-infected controls without (w/o) HL and healthy controls were used for immunophenotyping by polychromatic flow cytometry, including markers for activation, exhaustion and chemokine receptors.
Results: We identified significant differences in the CD4 + T cell count between HIV-infected individuals developing HL and HIV-infected matched controls within 1 year before cancer diagnosis. We observed substantial differences in the cellular phenotype mainly between healthy controls and HIV infection irrespective of HL. A number of markers tended to be different in Vδ1 and MAIT cells in HIV + HL + patients vs. HIV + w/o HL patients; notably, we observed significant differences for the expression of CCR5, CCR6 and CD16 between these two groups of HIV + patients.
Conclusion: TCR Vδ1 and MAIT cells in HIV-infected individuals developing HL show subtle phenotypical differences as compared to the ones in HIV-infected controls, which may go along with functional impairment and thereby may be less efficient in detecting and eliminating malignant cells. Further, our results support the potential of longitudinal CD4 + T cell count analysis for the identification of patients at higher risk to develop HL.
Databáze: MEDLINE
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