Characterization of extracellular vesicles isolated from types I, II and III strains of Toxoplasma gondii.

Autor: Quiarim TM; Laboratório de Biologia Molecular de Parasitas e Fungos, Instituto Adolfo Lutz, Sao Paulo, Brazil. Electronic address: tmquiarim@gmail.com., Maia MM; Laboratório de Biologia Molecular de Parasitas e Fungos, Instituto Adolfo Lutz, Sao Paulo, Brazil. Electronic address: m.marquesmaia.3@gmail.com., da Cruz AB; Laboratório de Biologia Molecular de Parasitas e Fungos, Instituto Adolfo Lutz, Sao Paulo, Brazil. Electronic address: abc_alle@hotmail.com., Taniwaki NN; Nucleo de Microscopia Eletrônica, Instituto Adolfo Lutz, Sao Paulo, Brazil. Electronic address: ntaniwak@hotmail.com., Namiyama GM; Nucleo de Microscopia Eletrônica, Instituto Adolfo Lutz, Sao Paulo, Brazil. Electronic address: ginamiyama@yahoo.com.br., Pereira-Chioccola VL; Laboratório de Biologia Molecular de Parasitas e Fungos, Instituto Adolfo Lutz, Sao Paulo, Brazil. Electronic address: pchioccola@gmail.com.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2021 Jul; Vol. 219, pp. 105915. Date of Electronic Publication: 2021 Apr 18.
DOI: 10.1016/j.actatropica.2021.105915
Abstrakt: This study investigated the participation extracellular vesicles (EVs) in Toxoplasma gondii-host interaction. EVs of three T. gondii strains (RH, ME-49 and VEG) were purified by chromatography and ELISA. Results of "nanoparticle tracking analysis" and scanning electron microscopy showed that RH strain released more EVs than other strains. Images of transmission electron microscopy showed that in beginning of incubation (culture medium), EVs were inside of tachyzoites preparing to be released. After 24 hours, they were largely produced inside tachyzoites and were released through plasma membrane. The parasite burden of mice infected with RH strain plus EVs was increased and with early death of 1-2 days compared of those that received only parasites. EV proteins of ME-49 and VEG strains were poorly reactive to sera of infected patients in imunoblot. However, those from RH strain were reactive against sera of patients with cerebral toxoplasmosis. EVs stimulated murine splenocytes caused similar production of IFN-γ and IL-10 levels. RH strain derived EVs stimulated more TNF-α than those stimulated by other strains. T. gondii and infected hosts can express the same miRNAs (miR-155-5p, miR-125b-5p, miR-423-3p). In conclusion, T. gondii derived EVs promote host-parasite interactions, modulate host immune responses, carry virulent factors and cause an imbalance in cellular immune response.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: