Neuropixels 2.0: A miniaturized high-density probe for stable, long-term brain recordings.

Autor:	Steinmetz NA; UCL Institute of Ophthalmology, University College London, London, UK. nick.steinmetz@gmail.com m.carandini@ucl.ac.uk harrist@janelia.hhmi.org.; Department of Biological Structure, University of Washington, Seattle, WA, USA., Aydin C; Neuroelectronics Research Flanders, Leuven, Belgium., Lebedeva A; Sainsbury Wellcome Centre, University College London, London, UK., Okun M; Centre for Systems Neuroscience and Department of Neuroscience, Psychology and Behaviour, University of Leicester, Leicester, UK.; UCL Queen Square Institute of Neurology, University College London, London, UK., Pachitariu M; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Bauza M; Sainsbury Wellcome Centre, University College London, London, UK., Beau M; Wolfson Institute for Biomedical Research, University College London, London, UK., Bhagat J; UCL Queen Square Institute of Neurology, University College London, London, UK., Böhm C; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Broux M; Neuroelectronics Research Flanders, Leuven, Belgium., Chen S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Colonell J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Gardner RJ; Kavli Institute for Systems Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway., Karsh B; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Kloosterman F; Neuroelectronics Research Flanders, Leuven, Belgium.; IMEC, Leuven, Belgium.; Vlaams Instituut voor Biotechnologie (VIB), Leuven, Belgium.; Brain and Cognition, KU Leuven, Leuven, Belgium., Kostadinov D; Wolfson Institute for Biomedical Research, University College London, London, UK., Mora-Lopez C; IMEC, Leuven, Belgium., O'Callaghan J; IMEC, Leuven, Belgium., Park J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Putzeys J; IMEC, Leuven, Belgium., Sauerbrei B; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., van Daal RJJ; ATLAS Neuroengineering, Leuven, Belgium.; Neuroelectronics Research Flanders, Leuven, Belgium.; Micro- and Nanosystems, KU Leuven, Leuven, Belgium., Vollan AZ; Kavli Institute for Systems Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway., Wang S; IMEC, Leuven, Belgium., Welkenhuysen M; IMEC, Leuven, Belgium., Ye Z; Department of Biological Structure, University of Washington, Seattle, WA, USA., Dudman JT; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Dutta B; IMEC, Leuven, Belgium., Hantman AW; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Harris KD; UCL Queen Square Institute of Neurology, University College London, London, UK., Lee AK; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Moser EI; Kavli Institute for Systems Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway., O'Keefe J; Sainsbury Wellcome Centre, University College London, London, UK., Renart A; Champalimaud Centre for the Unknown, Lisbon, Portugal., Svoboda K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Häusser M; Wolfson Institute for Biomedical Research, University College London, London, UK., Haesler S; Neuroelectronics Research Flanders, Leuven, Belgium.; Vlaams Instituut voor Biotechnologie (VIB), Leuven, Belgium., Carandini M; UCL Institute of Ophthalmology, University College London, London, UK. nick.steinmetz@gmail.com m.carandini@ucl.ac.uk harrist@janelia.hhmi.org., Harris TD; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. nick.steinmetz@gmail.com m.carandini@ucl.ac.uk harrist@janelia.hhmi.org.
Jazyk:	angličtina
Zdroj:	Science (New York, N.Y.) [Science] 2021 Apr 16; Vol. 372 (6539).
DOI:	10.1126/science.abf4588
Abstrakt:	Measuring the dynamics of neural processing across time scales requires following the spiking of thousands of individual neurons over milliseconds and months. To address this need, we introduce the Neuropixels 2.0 probe together with newly designed analysis algorithms. The probe has more than 5000 sites and is miniaturized to facilitate chronic implants in small mammals and recording during unrestrained behavior. High-quality recordings over long time scales were reliably obtained in mice and rats in six laboratories. Improved site density and arrangement combined with newly created data processing methods enable automatic post hoc correction for brain movements, allowing recording from the same neurons for more than 2 months. These probes and algorithms enable stable recordings from thousands of sites during free behavior, even in small animals such as mice. (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
Databáze:	MEDLINE
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